火针对膝骨关节炎大鼠软骨Wnt-4、β-catenin及MMP-13影响  

Effects of fire needling on Wnt-4,β-catenin,and MMP-13 in the cartilage of knee osteoarthritis rats

作　　者：蓝枰英 洪昆达 万甜 石欣悦 LANPingying;HONG Kunda;WAN Tian;SHI Xinyue(Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;The Second Affiliated Hospital of Fujian Medical University,Quanzhou 362000,China)

机构地区：[1]福建中医药大学,福州350122 [2]福建医科大学附属第二医院,泉州362000

出　　处：《上海针灸杂志》2025年第2期239-244,共6页Shanghai Journal of Acupuncture and Moxibustion

基　　金：福建医科大学附属第二医院博士启动基金(BS202006);福建省卫健委中青年骨干人才项目(2020GGA055)。

摘　　要：目的观察火针对膝骨关节炎(knee osteoarthritis,KOA)大鼠软骨组织形态以及Wnt-4、β-catenin、基质金属蛋白酶13(matrix metalloproteinase 13,MMP-13)蛋白表达的影响。方法将30只Sprague-Dawley(SD)大鼠随机分出11只为空白组,对剩余19只大鼠采用关节腔注射4%木瓜蛋白酶进行造模,在空白组和造模各随机抽取3只进行模型鉴定,余下的造模大鼠随机分成模型组和火针组,每组8只。火针组采用火针点刺双侧犊鼻和内膝眼穴治疗,空白组和模型组不进行任何治疗。观察3组大鼠体质量增长率、膝周径增长率和软骨外观。对3组大鼠膝关节软骨进行苏木精-伊红染色(hematoxylin-eosin staining,HE)并进行Mankin's评分比较。用Western blot检测软骨组织Wnt-4、β-catenin和MMP-13蛋白表达。结果造模后,造模大鼠体质量增长率低于空白组(P<0.05),膝周径增长率高于空白组(P<0.05),模型鉴定Mankin's评分高于空白组(P<0.05),表明造模成功。干预后,模型组体质量增长率低于空白组(P<0.05),火针组体质量增长率高于模型组(P<0.05);模型组膝周径增长率低于空白组(P<0.05),Mankin's评分高于空白组(P<0.05);火针组Mankin's评分低于模型组(P<0.05);模型组Wnt-4、β-catenin和MMP-13蛋白相对表达量均高于空白组(P<0.05),火针组Wnt-4、β-catenin和MMP-13蛋白相对表达量均低于模型组(P<0.05)。结论火针能抑制软骨退变,改善KOA症状,可能与调控Wnt/β-catenin信号通路下调MMP-13有关。Objective To observe the effects of fire needling on the morphology of cartilage tissue and the protein expression of Wnt-4,β-catenin,and matrix metalloproteinase 13(MMP-13)in knee osteoarthritis(KOA)rats.Method Thirty Sprague-Dawley(SD)rats were randomized into a blank control group(n=11)and a modeling group(n=19).Rats in the modeling group received intra-articular injection of 4%papain to establish KOA models.The two groups each randomly contributed 3 rats for model identification.The rest of the rats in the modeling group were randomly divided into a model group and a fire needling group,each consisting of 8 rats.Rats in the fire needling group were intervened by fire needling at bilateral Dubi(ST35)and Neixiyan(EX-LE5),and rats in the blank control and modeling groups did not receive any intervention.The three groups of rats were observed for the body mass growth rate,knee circumference increase rate,and the appearance of the cartilage.The knee cartilage of rats was treated with hematoxylin-eosin(HE)staining and compared using Mankin’s score among the three groups.The Western blot was used to determine the protein expression of Wnt-4,β-catenin,and MMP-13.Result Compared to the blank control group after modeling,the model rats had a smaller body mass growth rate(P<0.05),a higher knee circumference increase rate(P<0.05),and a higher Mankin’s score(P<0.05),suggesting the success of modeling.After the intervention,the model group showed a smaller body mass growth rate compared to the blank control group(P<0.05),and the fire needling group had a larger body mass growth rate than the model group(P<0.05);compared to the blank control group,the model group had a smaller knee circumference increase rate(P<0.05)and a higher Mankin’s score(P<0.05);the fire needling group had a lower Mankin’s score than the model group(P<0.05);the relative protein expression levels of Wnt-4,β-catenin,and MMP-13 were higher in the model group than in the blank control group(P<0.05)and were lower in the fire needling group than

关 键 词：火针疗法 骨关节炎 膝 穴 犊鼻 穴 内膝眼 大鼠 

分 类 号：R2-03[医药卫生—中医学]