基于非靶向代谢组学分析不同饲喂方式下翘嘴鳜肠道代谢的差异  

Untargeted metabolomics analysis of differences in intestinal metabolism of Siniperca chuatsi with different feeding patterns

作　　者：方婷 赵秀侠[1,2,3] 高娜 卢文轩[1,2,3] 梁阳阳 汪翔 蒋阳阳[2,3] 孙雯 严香兰 崔凯[1,2,3] FANG Ting;ZHAO Xiuxia;GAO Na;LU Wenxuan;LIANG Yangyang;WANG Xiang;JIANG Yangyang;SUN Wen;YAN Xianglan;CUI Kai(Hefei Comprehensive Experimental Station of National Featured Freshwater Fish Industry Technology System,Hefei 230031,China;Fishery Research Institute,Anhui Academy of Agricultural Sciences,Hefei 230031,China;Anhui Province Key Laboratory of Aquaculture&Stock Enhancement,Hefei 230031,China;Dongzhi County Dadukou Town Aquatic Technology Promotion Station,Chizhou 247200,Anhui,China)

机构地区：[1]国家特色淡水鱼产业技术体系合肥综合试验站,合肥230031 [2]安徽省农业科学院水产研究所,合肥230031 [3]水产增养殖安徽省重点实验室,合肥230031 [4]东至县大渡口镇水产技术推广站,安徽池州247200

出　　处：《淡水渔业》2025年第1期40-48,共9页Freshwater Fisheries

基　　金：国家现代农业产业技术体系(CARS-46);安徽省现代农业产业技术体系建设专项(皖农科函[2021]711号);安徽省农业科学院青年英才计划项目(QNYC-202108)。

摘　　要：采用液相色谱-质谱联用非靶向代谢组学方法,分析了配合饲料和饵料鱼两种饲喂方式下翘嘴鳜(Siniperca chuatsi)肠内容物代谢谱的差异,通过主成分分析与数据库比对,筛选差异代谢物及代谢通路,为鳜鱼养殖及其生理代谢研究提供参考。结果显示:通过代谢组学分析,两组间肠道代谢谱存在显著差异,在正离子和负离子模式下共筛选出735种差异代谢物,其中43种代谢物在饲料鳜组显著上调,7种代谢物在饲料鳜组显著下调;KEGG通路富集分析表明差异代谢物主要富集在ABC转运、组氨酸代谢、精氨酸-脯氨酸代谢、胆汁分泌、嘧啶代谢、核苷酸代谢等通路中。研究结果初步表明,配合饲料显著改变了鳜的肠道代谢特征。Siniperca chuatsi is a typical carnivorous fish,of which compound feed has been successfully used to substitute forage fish in its intensive farming.However,the difference in its intestinal metabolism under different diets is still unknown.A metabolomics study was performed on the gut content of S.chuatsi fed with compound feed and forage fish to investigate the dietary effects on the intestinal metabolism of S.chuatsi.A non-targeted metabolomic method using liquid chromatography-mass spectrometry(LC-MS)was applied to investigate the gut metabolic profiles of S.chuatsi,thereafter,principle component analysis and database comparison were used to screen differential metabolites and metabolic pathways.The results showed significant differences in gut content metabolite profiles between the compound feed group and the forage fish group.A total of 735 different metabolites were screened under positive and negative ion modes,among which 43 were significantly up-regulated in the compound feed group and 7 were significantly down-regulated in the compound feed group.KEGG pathway enrichment analysis showed that differential metabolites were mainly concentrated in ABC transport,histidine metabolism,arginine-proline metabolism,bile secretion,pyrimidine metabolism,nucleotide metabolism and other pathways.Results showed that the compound feed significantly changed the intestinal metabolic characteristics of S.chuatsi.The results of this study can provide scientific reference for the intestinal metabolism of S.chuatsi and its adaptation mechanism to compound feed.

关 键 词：翘嘴鳜(Siniperca chuatsi) 非靶向代谢组学 饲喂方式 差异代谢通路 

分 类 号：S917.4[农业科学—水产科学]