小檗碱通过调控CaN-NFAT信号通路缓解柔红霉素诱导的心肌细胞损伤的研究  

Reducing effect of berberine on daunorubicin-induced cardiomyocyte injury by regulating CaN-NFAT signaling pathway

作　　者：庞涵睿 韩轩茂 蔺雪峰[2] PANG Hanrui;HAN Xuanmao;LIN Xuefeng(Baotou Medical College,Inner Mongolia University of Science and Technology,Baotou 014040,China;The First Ward of Cardiology Department,The First Affiliated Hospital of Baotou Medical College,Inner Mongolia University of Science and Technology)

机构地区：[1]内蒙古科技大学包头医学院,内蒙古包头014000 [2]内蒙古科技大学包头医学院第一附属医院心内科一病区

出　　处：《包头医学院学报》2025年第1期43-48,共6页Journal of Baotou Medical College

基　　金：内蒙古自治区自然科学基金项目(2023MS08040)。

摘　　要：目的:探究小檗碱(berberine,BBR)对柔红霉素(daunorubicin,DNR)损伤心肌细胞的保护效应,以及CaN-NFAT通路在其中的作用。方法:将生长良好的H9C2心肌细胞按简单随机分组方法分为4组:CON组、DNR组、DNR+BBR组和DNR+VIVIT(通路抑制剂)组,显微镜观察H9C2心肌细胞形态;CCK-8法检测H9C2心肌细胞存活率;生化试剂盒检测H9C2心肌细胞损伤情况;Western Blot法检测NFATc4蛋白及凋亡蛋白Bax、Bcl-2表达。结果:(1)显微镜下细胞形态:与CON组相比,DNR组心肌细胞数量减少;与DNR组相比,DNR+BBR组、DNR+VIVIT组心肌细胞数量增加;(2)CCK-8法测细胞存活率:与CON组相比,DNR组心肌细胞存活率明显下降(P<0.05),与DNR组相比,DNR+BBR组、DNR+VIVIT组心肌细胞存活率上升(P<0.05);(3)肌酸激酶(creatine kinase,CK)含量及乳酸脱氢酶(lactate dehydrogenase,LDH)释放量:①与CON组相比,DNR组CK活性升高(P<0.05);与DNR组相比,DNR+BBR、DNR+VIVIT组CK含量降低(P<0.05);②与CON组相比,DNR组的LDH释放量升高(P<0.05);与DNR组相比,DNR+BBR、DNR+VIVIT组LDH释放量降低(P<0.05);(4)Western Blot:①与CON组相比,DNR组Bax表达、Bax/Bcl-2比值及NFATc4表达升高而Bcl-2表达下降(P<0.05);②与DNR组相比,DNR+BBR组、DNR+VIVIT组Bax表达、Bax/Bcl-2及NFATc4表达下降而Bcl-2表达升高(P<0.05)。结论:BBR可能通过调控CaN-NFAT信号通路抑制DNR诱导的心肌细胞损伤,减少细胞凋亡。Objective:To investigate the protective effect of berberine(BBR)on daunorubicin(DNR)-induced cardiomyocyte injury and the role of CaN-NFAT pathway.Methods:H9C2 cardiomyocytes were randomly divided into 4 groups:CON group,DNR group,DNR+BBR group and DNR+VIVIT(pathway inhibitor)group.The morphology of H9C2 cardiomyocytes was observed by microscope.CCK-8 method was used to detect the survival rate of H9C2 cardiomyocytes.The injury of H9C2 cardiomyocytes was detected by biochemical kit.Western Blot was used to detect the expression of NFATc4 protein and apoptotic proteins Bax and Bcl-2.Results:(1)Cell morphology under the microscope:compared with the CON group,the number of cardiomyocytes in the DNR group decreased.Compared with the DNR group,the number of cardiomyocytes in the DNR+BBR group and the DNR+VIVIT group increased.(2)CCK-8 assay:compared with CON group,the survival rate of cardiomyocytes in DNR group was significantly decreased(P<0.05).Compared with DNR group,the survival rate of cardiomyocytes in DNR+BBR group and DNR+VIVIT group was increased(P<0.05).(3)Creatine kinase(CK)content and lactate dehydrogenase(LDH)release:①Compared with the CON group,the CK activity of the DNR group increased(P<0.05).Compared with the DNR group,the CK content in the DNR+BBR and DNR+VIVIT groups decreased(P<0.05).②Compared with the CON group,the LDH release in the DNR group was increased(P<0.05).Compared with the DNR group,the LDH release in the DNR+BBR and DNR+VIVIT groups was decreased(P<0.05).(4)Western Blot:①Compared with the CON group,the expression of Bax,Bax/Bcl-2 ratio and NFATc4 in the DNR group increased,while the expression of Bcl-2 decreased(P<0.05).②Compared with DNR group,the expression of Bax,Bax/Bcl-2 and NFATc4 in DNR+BBR group and DNR+VIVIT group decreased,while the expression of Bcl-2 increased(P<0.05).Conclusion:BBR may inhibit DNR induced cardiomyocyte injury and reduce apoptosis by regulating CaN-NFAT signaling pathway.

关 键 词：小檗碱 柔红霉素 心肌损伤 CaN-NFAT信号通路 

分 类 号：R73[医药卫生—肿瘤]