金黄色葡萄球菌脂蛋白在诱导小鼠巨噬细胞炎性反应中的作用  

The Role of S.aureus Lipoprotein in the Induction of Inflammatory Responses of Mouse Macrophages

作　　者：刘博[1] 巩志国 哈斯苏荣[1] LIU Bo;GONG Zhi-guo;HASI Surong(Inner Mongolia Agricultural University,Inner Mongolia,Hohhot,010018,China)

机构地区：[1]内蒙古农业大学,内蒙古呼和浩特010018

出　　处：《动物医学进展》2025年第2期16-22,共7页Progress In Veterinary Medicine

基　　金：中央引导地方科技发展基金项目(2022ZY0200);国家自然科学基金项目(32060815)。

摘　　要：金黄色葡萄球菌(S.aureus)是导致哺乳动物感染性疾病的重要革兰氏阳性病原菌,其侵入宿主机体后能够在短时间内激活天然免疫系统,诱导炎症反应的发生。论文探究了细菌菌体成分对免疫细胞炎性反应的诱导作用。通过实时荧光定量PCR、Western blot和ELISA方法,分析了S.aureus脂蛋白在诱导小鼠巨噬细胞模式识别受体TLR2表达、炎性细胞因子(TNF-α、IL-1β和IL-6)和趋化因子(RANTES)分泌、炎性信号通路激活、前列腺素E_(2)(PGE 2)分泌中的作用。结果表明,与S.aureus SA113野生株和脂蛋白表达回补株相比,S.aureus脂蛋白表达缺失株诱导巨噬细胞TLR_(2)表达的能力处于较低水平(P<0.05)。同时,细菌脂蛋白是S.aureus感染过程中诱导巨噬细胞炎性信号通路激活,进而导致炎性细胞因子、趋化因子和炎症介质PGE _(2)分泌的重要免疫活性物质。此外,细胞脂蛋白对S.aureus在巨噬细胞中的存活发挥了重要作用。表明细菌脂蛋白对S.aureus诱导巨噬细胞炎性反应具有重要作用,可能是参与S.aureus感染性疾病进程发展的关键菌体成分。Staphylococcus aureus is a significant Gram-positive pathogen responsible for infectious diseases in mammals.Upon invading the host,it promptly activates the innate immune system,triggering inflammation.In this study,we investigated the induction effect of bacterial components on the inflammatory response of immune cells.The real-time PCR,Western blot and ELISA were used to analyze the roles of S.aureus lipoproteins in eliciting the expression of the pattern recognition receptor TLR_(2),secretion of inflammatory cytokines(TNF-α,IL-1β,and IL-6),chemokines(RANTES),activation of inflammatory signaling pathways,and secretion of prostaglandin E_(2)(PGE_(2))in mouse macrophages.The findings demonstrated that the S.aureus lipoprotein expression-deficient strain exhibited a diminished ability to induce TLR_(2)expression in macrophages compared to the wild-type S.aureus SA113 strain and its lipoprotein expression complement strain(P<0.05).Bacterial lipoproteins emerged as crucial immunoreactive compounds,driving the activation of macrophage inflammatory signaling pathways during S.aureus infection.This activation resulted in the secretion of inflammatory cytokines,chemokines,and the inflammatory mediator PGE_(2).Additionally,lipoproteins played a pivotal role in the survival of S.aureus within macrophages.These results signify the essential role of bacterial lipoproteins in macrophage-induced inflammatory responses to S.aureus.These lipoproteins may serve as key components influencing the development of S.aureus infectious diseases.

关 键 词：金黄色葡萄球菌 细菌脂蛋白 巨噬细胞 炎性反应 细胞内存活 

分 类 号：S859.7[农业科学—临床兽医学]