鼠伤寒沙门氏菌rpoE基因克隆和生物信息学分析  

Cloning and Bioinformatics Analysis of rpoE Gene of S.typhimurium

作　　者：何金娇 贾凯凯 靳明霞 赵万里 殷俊磊 仇书兴 孙国鹏[1] HE Jin-jiao;JIA Kai-kai;JIN Ming-xia;ZHAO Wan-li;YIN Jun-lei;QIU Shu-xing;SUN Guo-peng(Henan Engineering Laboratory of Molecular Diagnosis of Animal Diseases,Xinxiang University,Xinxiang,Henan,453003,China;College of Life Sciences,Henan Normal University,Xinxiang,Henan,453007,China;College of Medicine,Xinxiang University,Xinxiang,Henan,453003,China)

机构地区：[1]新乡学院动物疫病分子诊断河南省工程实验室,河南新乡453003 [2]河南师范大学生命科学学院,河南新乡453007 [3]新乡学院医学院,河南新乡453003

出　　处：《动物医学进展》2025年第2期30-34,共5页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31672540);河南省高等学校重点科研项目(23A310032);新乡学院博士科研启动资助项目(1366020118);新乡学院校级一流课程建设项目(1205522212)。

摘　　要：旨在克隆鼠伤寒沙门氏菌的rpoE基因,通过生物信息学在线预测软件对rpoE基因编码的RpoE蛋白进行生物信息学分析。以禽源鼠伤寒沙门氏菌基因组DNA为模板,PCR扩增rpoE基因,成功测序后通过DNAStar软件对RpoE蛋白进行B细胞抗原表位分析,MEGA5.2软件对RpoE蛋白进行同源性分析和系统进化树构建。结果表明,成功克隆的鼠伤寒沙门氏菌rpoE基因全长576 bp;抗原表位分析显示RpoE蛋白表面存在12个优势B细胞抗原表位位点;同源性分析和进化树构建结果表明鼠伤寒沙门氏菌RpoE蛋白与肠炎沙门氏菌的同源性最高,并与其处于同一分支内,可为后续研究鼠伤寒沙门氏菌的致病机制提供基础信息。The objective of this study was to clone the rpoE gene of Salmonella typhimurium and analyze the RpoE protein encoded by the rpoE gene using bioinformatics online prediction software,primarily for epitope prediction,homology analysis,and phylogenetic tree construction.Genomic DNA from S.typhimurium was extracted,and the rpoE gene was amplified through PCR.The B-cell epitopes of RpoE protein were analyzed using DNAstar software.Homology analysis and phylogenetic tree construction of RpoE protein were conducted using MEGA5.2 software.The results showed that the length of the rpoE gene in S.typhimurium was 576bp.Epitope analysis demonstrated that there were 12 prominent B cell epitope sites on the surface of RpoE protein.Homology analysis and phylogenetic tree construction results indicated that the RpoE protein in S.typhimurium exhibited highest similarity with S.enteritis,and belonged to same branch as it did.This study provides pertinent information for further research on the pathogensis and treatment strategies related to S.typhimurium.

关 键 词：鼠伤寒沙门氏菌 rpoE基因 克隆 生物信息学 

分 类 号：S852.612[农业科学—基础兽医学]