健脾养肝丸对肝郁脾虚型IBS-D模型大鼠肥大细胞活化的影响  

Effect of Jianpi Yanggan Pill on the activation of mast cells in rats withliver-stagnation and spleen-deficiency IBS-D model

作　　者：朱婷 陈欣 赵鑫 牛智琴 何瑞 栾云鹏 李襄 王华宁 马可 Zhu Ting;Chen Xin;Zhao Xin;Niu Zhi-qin;He Rui;Luan Yun-peng;Li Xiang;Wang Hua-ning;Ma Ke(Department of The First Affiliated Hospital of YunNan University of TCM,Kunming 650500,China)

机构地区：[1]云南中医药大学第一附属医院,云南昆明650500

出　　处：《北方药学》2024年第11期12-13,40,共3页Journal of North Pharmacy

基　　金：云南省科技厅中医联合专项-面上项目(202201AG070034);云南省科技厅中医联合专项-面上项目(202101AG070370);云南省教育厅科学研究基金资助项目(2023Y0484);云南中医药大学-云南中医药大学第一附属医院联合基金项目(XYLH202001)。

摘　　要：目的:观察健脾养肝丸对腹泻型肠易激综合征(diarrhea-type irritable bowel syndrome, IBS-D)模型大鼠结肠肥大细胞(mast cell, MC)的影响。方法:SD大鼠24只,随机分为空白组、模型组、健脾养肝丸组和匹维溴铵组,采用乙酸灌肠联合束缚应激法制备IBS-D大鼠模型,给药14d后进行AWR评分,电镜观察结肠MC的变化,免疫荧光检测结肠MC标记物tryptase的表达。结果:与模型组相比,健脾养肝丸组大鼠AWR评分明显降低,观察免疫荧光染色结肠组织内Tryptase的表达,健脾养肝丸能降低模型组大鼠结肠MC数量,电镜观察IBS-D大鼠MC颗粒密度降低,胞内出现边缘空隙。结论:健脾养肝丸可能通过调控MC的活化从而对IBS-D发挥治疗作用。Objective:To observe the effects of Jianpiyanggan pills(JPYGW)on colon mast cells(MC)in rats with diarrhea type irritable bowel syndrome(IBS D).Methods:Twenty four SD rats were randomly divided into blank group,model group,JPYGW group and Piverium bromide group.The IBS D rat models were prepared by acetic acid irrigation combined with binding stress method.AWR score was performed after 14 days of administration,the changes of colon MC were observed by electron microscope,and the expression of colon MC marker tryptase was detected by immunofluorescence.Results:Compared with them odel group,the AWR score of the rats in the JPYGW group was significantly decreased,the expression of Tryptase in the colon tissues of the rats in the model group was observed by immunofluorescence staining,and the number of MC particles in the colon of the rats in the model group was significantly decreased by JPYGW.Conclusion:JPYGW may play a therapeutic role on IBS D by regulating the activation of MC.

关 键 词：健脾养肝丸 腹泻型肠易激综合征 肥大细胞 类胰蛋白酶 

分 类 号：R965[医药卫生—药理学]