抗生素饲喂小鼠对发热伴血小板减少综合征病毒在蜱虫共吸血传播中的影响  

Effect of antibiotic feeding in mice on the cofeeding transmission of fever with thrombocytopenia syndrome virus in ticks

作　　者：陈京晶 陈晨 周祥 袁传飞 夏乾峰 CHEN Jingjing;CHEN Chen;ZHOU Xiang;YUAN Chuanfei;XIA Qianfeng(School of Tropical Medicine,Hainan Medical University,Haikou 571199,China)

机构地区：[1]海南医科大学热带医学院,海南海口571199

出　　处：《海南医科大学学报》2025年第3期161-168,共8页Journal of Hainan Medical University

基　　金：国家自然科学基金项目(82102433)。

摘　　要：目的:发热伴血小板减少综合征病毒(severe fever with thrombocytopenia syndrome virus,SFTSV)是一种新发的蜱传病原体。探讨小鼠肠道菌群在SFTSV传播中的作用。方法:通过抗生素治疗和微生物再定殖对昆明小鼠肠道菌群进行调控。然后将感染SFTSV的长角血蜱成蜱和未感染的若蜱共同吸血小鼠。采用实时荧光定量PCR(qPCR)方法检测饱血若蜱体内SFTSV水平。结果:将粪便均匀涂抹在LB琼脂平板上,证实肠道细菌的改变。与对照组和再定殖组相比,抗生素治疗组的微生物群落多样性较单一。在共吸血传播模型中,qPCR分析显示抗生素治疗组的病毒载量较对照组和再定殖组有所增加。结论:小鼠肠道菌群可抑制SFTSV的传播。研究结果为进一步探索SFTSV传播中宿主肠道功能菌提供了基础。Objective:To investigate the role of mouse gut microbiota in the transmission of severe fever with thrombocytopenia syndrome virus(SFTSV),a newly emerging tick-borne pathogen.Methods:The gut microbiota of Kunming mice was manipulated by antibiotics treatment and microbial recolonization.Then,infected adults and uninfected nymphs were co-fed on the mice.The SFTSV levels were determined in the engorged nymphs using quantitative real-time PCR(qPCR)analysis.Results:The alteration of gut bacteria was confirmed by plating the homogenate of faeces onto Luria-Bcrtani(LB)agar plates.Microbial community displayed limited variety in the antibiotic treatment group compared to the control group and the recolonization group.In the model of co-feeding transmission,qPCR analysis revealed that the viral load increased in the antibiotic treatment group compared to the control group and the recolonization group.Conclusion:Mouse gut microbiota can inhibit the transmission of SFTSV.The results provide a basis for further exploration of functional bacteria of host gut in SFTSV transmission.

关 键 词：严重发热伴血小板减少综合征病毒 长角血蜱 肠道 微生物 

分 类 号：R373[医药卫生—病原生物学]