分析应用芯片检测高糖和葡萄糖胺诱导的基因表达对肾系膜细胞氧化应激的预警意义  

Analysis of early warning significance of microarray detection of gene expression induced by high glucose and glucosamine for oxidative stress in renal mesangial cells

作　　者：余洁玲[1] 梁洁如 钟明芝 YU Jie-ling;LIANG Jie-ru;ZHONGMing-zhi(Laboratory Department,Houjie Hospital in Dongguan,Dongguan 523945,China)

机构地区：[1]东莞市厚街医院检验科,523945 [2]东莞市厚街医院内分泌科,523945

出　　处：《中国实用医药》2025年第2期76-79,共4页China Practical Medicine

基　　金：2023年东莞市社会发展科技项目(项目编号:20231800903042)。

摘　　要：目的探讨应用芯片检测高糖和葡萄糖胺诱导的基因表达评估肾系膜细胞氧化应激的预警意义和价值。方法选择120例糖尿病肾病(DN)患者为观察组,另选同时间段120例健康体检者为对照组。采用芯片检测高糖和葡萄糖胺诱导的基因表达,检测两组细胞氧化应激及肾功能指标。比较两组谷氨酰胺果糖6-磷酸氨基转移酶(GFAT)、细胞外基质(ECM)、转化生长因子α、泛素样结构域蛋白成员1、硫氧还蛋白(TRX)、谷胱甘肽过氧化酶1、代谢型谷氨酸受体1、70 kDa热休克蛋白5基因表达水平,细胞氧化应激[还原型谷胱甘肽(GSH)与氧化型谷胱甘肽(GSSG)的比值(GSH/GSSG)、活性氧自由基]及肾功能指标[尿微量白蛋白、肌酐(Cr)、视黄醇结合蛋白(RBP)、β_(2)微球蛋白(β_(2)-MG)、胱抑素C(Cys C)、尿素氮(BUN)];采用Pearson线性相关分析高糖和葡萄糖胺诱导的基因表达与肾系膜细胞氧化应激的相关性。结果观察组GFAT、ECM、转化生长因子α、泛素样结构域蛋白成员1、TRX、谷胱甘肽过氧化酶1、代谢型谷氨酸受体1、70 kDa热休克蛋白5基因表达水平分别为(1.57±0.32)、(1.61±0.35)、(1.43±0.29)、(1.44±0.32)、(1.63±0.37)、(1.32±0.24)、(1.54±0.27)、(1.58±0.33),高于对照组的(0.63±0.11)、(0.75±0.17)、(0.46±0.07)、(0.78±0.19)、(0.54±0.12)、(0.68±0.14)、(0.43±0.09)、(0.84±0.21)(P<0.05)。观察组GSH/GSSG(6.67±0.11)低于对照组的(9.02±0.07),活性氧自由基(2853.73±62.16)μmol/L、尿微量白蛋白(42.64±3.26)mg/24 h、Cr(213.85±89.41)μmol/L、RBP(114.29±16.25)mg/L、β_(2)-MG(3.05±0.21)mg/L、Cys C(5.31±0.61)mg/L、BUN(12.13±2.14)mmol/L高于对照组的(2325.91±55.79)μmol/L、(19.21±1.52)mg/24 h、(91.19±14.29)μmol/L、(40.24±8.51)mg/L、(1.32±0.06)mg/L、(1.03±0.23)mg/L、(4.39±0.84)mmol/L(P<0.05)。Pearson线性相关分析结果显示:DN患者GFAT、ECM、转化生长因子α、泛素样结构域蛋白成员1、TRX、谷胱甘肽过�Objective To explore the early warning significance and value of microarray detection of gene expression induced by high glucose and glucosamine in evaluating oxidative stress in renal mesangial cells.Methods 120 patients with diabetic nephropathy(DN)were selected as the observation group;another 120 healthy examinees during this time period were selected as the control group.The gene expression induced by high glucose and glucosamine was detected by microarrays,and the cellular oxidative stress and renal function indicators in the two groups were detected.Comparison was made on expression levels of fructose-6-phosphate amidotransferase(GFAT),extracellular matrix(ECM),transforming growth factor-α,ubiquitin-like domain member 1,thioredoxin(TRX),glutathione peroxidase 1,metabolic glutamate receptor 1,heat shock 70 kDa protein 5,cellular oxidative stress[ratio of reduced glutathione(GSH)to oxidized glutathione(GSSG)(GSH/GSSG),reactive oxygen species]and renal function indicators[urinary microalbumin,creatinine(Cr),retinol binding protein(RBP),β_(2)-microglobulin(β_(2)-MG),cystatin C(Cys C),blood urea nitrogen(BUN)]between the two groups.Pearson linear correlation analysis was used to investigate the correlation between high glucose and glucosamine induced gene expression and oxidative stress in renal mesangial cells.Results The gene expression levels of GFAT,ECM,transforming growth factor-α,ubiquitin-like domain member 1,TRX,glutathione peroxidase 1,metabolic glutamate receptor 1,and heat shock 70 kDa protein 5 in the observation group were(1.57±0.32),(1.61±0.35),(1.43±0.29),(1.44±0.32),(1.63±0.37),(1.32±0.24),(1.54±0.27),and(1.58±0.33),which were higher than(0.63±0.11),(0.75±0.17),(0.46±0.07),(0.78±0.19),(0.54±0.12),(0.68±0.14),(0.43±0.09),and(0.84±0.21)in the control group(P<0.05).GSH/GSSG of(6.67±0.11)in the observation group was lower than(9.02±0.07)in the control group;in the observation group,the reactive oxygen species was(2853.73±62.16)μmol/L,the urinary microalbumin was(42.64±3.26)m

关 键 词：芯片检测 高糖 葡萄糖胺 基因表达 肾系膜细胞 氧化应激 预警意义 

分 类 号：R692.9[医药卫生—泌尿科学] R587.2[医药卫生—外科学]