高脂喂养对SD雄性大鼠前列腺和精囊腺花生四烯酸代谢的影响  

Effects of high-fat diet on arachidonic acid metabolism in prostate and seminal vesicle of SD male rats

作　　者：代良 韩丽[2] 刘永杰 刘畅 马文欣 马会明[4] DAI Liang;HAN Li;LIU Yongjie;LIU Chang;MA Wenxin;MA Huiming(Reproductive Medical Center,Yinchuan Women and Children Health Care Hospital,Yinchuan 750001,China;Department of Anesthesia and Perioperative Medicine,General Hospital of Ningxia Medical University,Yinchuan 750004,China;School of Basic Medicine Sciences,Ningxia Medical University,Yinchuan 750004,China;Key Laboratory of Ningxia Medical University Fertility Maintenance,Ministry of Education,Yinchuan 750004,China)

机构地区：[1]银川市妇幼保健院生殖中心,宁夏银川750001 [2]宁夏医科大学总医院麻醉与围手术医学科,宁夏银川750004 [3]宁夏医科大学基础医学院,宁夏银川750004 [4]宁夏医科大学生育力保持教育部重点实验室,宁夏银川750004

出　　处：《西部医学》2025年第2期175-179,共5页Medical Journal of West China

基　　金：宁夏自然科学基金资助项目(2022AAC03744);宁夏重点研发计划项目(2022BEG03083)。

摘　　要：目的 探讨高脂喂养对SD雄性大鼠前列腺和精囊腺花生四烯酸(AA)代谢的影响。方法 选择24只45 d龄SD雄性大鼠,分为对照组、肥胖组和转换组3组,每组8只。其中对照组给予普通饲料喂养,肥胖组给予45%高脂饲料喂养,转换组给予先高脂饲料喂养2月后转为普通饲料,3组均共喂养3个月。采用ELISA法测定磷脂(PL)、分泌型磷脂酶A2(sPLA2)、AA、5脂氧化酶(5-LOX)、环氧化酶(COX)1、COX2、白三烯B4(LTB4)、前列腺素(PG)E1、PGF_(2α)、白细胞介素(IL)1、IL-6、肿瘤坏死因子α(TNF-α)、睾酮(T)、雌激素(E_(2))的浓度。统计分析3组间各指标的差异性,分析AA代谢相关物质之间的相关性。结果 前列腺的LTB4、IL-6、TNF-α肥胖组和转换组均显著高于对照组(P<0.05),精囊腺的E_(2)肥胖组和转换组均显著高于对照组(P<0.05)。前列腺中,体重与AA呈正相关,AA代谢网络各指标间均为正相关,而PGE1与IL-6呈负相关(均P<0.05)。精囊腺中,体重与COX1、5-LOX及E_(2)呈正相关,除COX2与PL和COX1呈负相关,其余AA代谢网络各指标间均呈正相关(均P<0.05)。结论 高脂饮食会影响SD雄性大鼠的AA代谢,在前列腺中会产生明确的增量效应,影响前列腺的功能状态,而在精囊腺中主要表现为COX1、COX2及5-LOX三者间的此消彼长趋势。Objective To explore the effect of high-fat diet on arachidonic acid metabolism in prostate and seminal vesicle of SD male rats.Methods Twenty four 45 day old SD male rats were selected and divided into control group,obesity group and conversion group,with 8 rats in each group.The control group was fed with normal diet,the obese group was fed with 45% high-fat diet,and the conversion group was fed with high-fat diet for 2 months and then converted to normal diet.The three groups were fed with high-fat diet for 3 months,and determine the concentration of phospholipid(PL),secretory phospholipase A2(sPLA2),arachidonic acid(AA),5 lipoxygenase(5-LOX),cyclooxygenase(COX)1,COX2,leukotriene B4(LTB4),prostaglandin(PG)E1,PGF_(2α),Interleukin(IL)1,IL-6,tumor necrosis factor α(TNF-α),testosterone(T) and estrogen(E_(2)).Analyzed the difference of each index among the control group,obesity group and conversion group,.Results The levels of LTB4,IL-6 and TNF-α in prostate obesity group and transition group were significantly higher than those in control group(P<0.05),and the levels of E_(2) in seminal vesicle gland obesity group and transition group were significantly higher than those in control group(P<0.05).In prostate,body weight was positively correlated with AA,all indexes of AA metabolic network were positively correlated,and PGE1 was negatively correlated with IL-6(P<0.05).In seminal vesicle gland,body weight was positively correlated with COX1,5-LOX and E_(2),except COX2 was negatively correlated with PL and COX1,other AA metabolic network indicators were positively correlated(P<0.05).Conclusion High-fat diet can affect the AA metabolism of SD male rats,and has a clear incremental effect on the prostate gland,affecting the functional state of the prostate,and the ebb and flow trend of COX1,COX2 and 5-LOX in the seminal vesicles.

关 键 词：高脂 SD雄性大鼠 前列腺 精囊腺 花生四烯酸 

分 类 号：R697.3[医药卫生—泌尿科学] R697.4[医药卫生—外科学]