基于高密度遗传图谱发掘杂草稻早抽穗QTL  

Exploring QTL for Early Heading of Weedy Rice Based on High-density Genetic Map

作　　者：李转 梁铖玮 于晓玉 韩雨晴 崔娟 赵雪 孙健[1] 陈温福[1] LI Zhuan;LIANG Chengwei;YU Xiaoyu;HAN Yuqing;CUI Juan;ZHAO Xue;SUN Jian;CHEN Wenfu(Rice Research Institute,Shenyang Agricultural University,Shenyang 110161,China)

机构地区：[1]沈阳农业大学水稻研究所,沈阳110161

出　　处：《沈阳农业大学学报》2025年第1期1-8,共8页Journal of Shenyang Agricultural University

基　　金：国家自然科学基金面上项目(32372107)。

摘　　要：[目的]水稻(Oryza sativa L.)抽穗期是由多基因调控的数量性状,影响水稻产量和品质,决定了水稻适应种植区域和分布范围,抽穗标志着水稻由营养生长转入生殖生长。杂草稻作为一类早熟型种质资源,其抽穗期往往提前于栽培稻,然而其遗传机制尚未被充分阐明。[方法]基于早抽穗的粳型杂草稻WR04-6和晚抽穗的籼稻七山占构建的包含168个体的重组自交系群体,结合高密度的SNP遗传图谱应用完备复合区间作图的加性效应模型进行抽穗期QTL定位。[结果]在以LOD值为2.5的阈值线之上,鉴定到唯一1个显著调控杂草稻早抽穗的QTL,区间内包含70个基因,基于组织特异性表达谱分析与基因注释推测LOC_Os01g08700即OsGI作为目标候选基因,进行后续的验证和生物信息学分析,OsGI不同血缘单倍型之间差异显著,位于细胞核内,在籼稻和粳稻之间有明显的亚种间分化。[结论]在水稻OsGI-Hd1-Hd3a/RFT1的抽穗期调控途径中,Hd1变异调控水稻抽穗期和分布被广泛报导,而本研究中发现,上游的生物钟基因OsGI在杂草稻中的自然变异,可能影响杂草稻的适应性分布。研究结果为杂草稻早抽穗的分子遗传调控机制提供了新视角,也为杂草稻早熟基因资源应用于栽培稻遗传改良贡献了新的靶点。[Objective]The heading date of rice(Oryza sativa L.)is a quantitative trait regulated by multiple genes,which affects the yield and quality of rice,and determines the planting area and distribution range,heading marks the transformation of rice from vegetative growth to reproductive growth.Weedy rice(Oryza sativa f.spontanea)as a kind of early heading germplasm resources,its heading date is often earlier than that of cultivar,while its genetic mechanism has not been fully elucidated.[Methods]In this study,a recombinant inbred line population consisting of 168 individuals was constructed based on early heading japonica weedy rice WR04-6 and late heading indica rice Qishanzhan.A high-density SNP genetic map was used to apply a inclusive composite interval mapping with additive effect model for QTL mapping at heading stage.[Results]Above the threshold line with a LOD score of 2.5,the only QTL regulating early heading of weedy rice was identified,containing 70 genes in the interval.Based on tissue-specific expression profile analysis and gene annotation,LOC_Os01g08700,also known as OsGI,was speculated as the target candidate gene.Subsequent verification and bioinformatics analysis showed significant differences between different haplotypes of OsGI,which located in the nucleus of the cell and exhibiting significant inter subspecies differentiation between indica and japonica rice.[Conclusion]In the heading regulation pathway of rice OsGI-Hd1-Hd3a/RFT1,Hd1 variation has been widely reported to regulate the heading stage and distribution of rice.However,this study found that the natural variation of the upstream circadian clock gene OsGI in weedy rice may affect its adaptive distribution.This study provides a new perspective on the molecular genetic regulation mechanism of early heading in weedy rice,and also contributes new a targets for the application of early heading gene resources in cultivated rice genetic improvement.

关 键 词：抽穗期 杂草稻 遗传图谱 QTL定位 生物钟 

分 类 号：S511[农业科学—作物学]