水稻不同生育期根际溶磷菌群落特征  

Characteristics of phosphorus solubilizing bacterial communities in the rhizosphere of rice at different growth stages

作　　者：吴书琴 胡敏 薛银刚[1] 于焕云 严松[3] 汤珊珊 顾昊宇 WU Shuqin;HU Min;XUE Yingang;YU Huanyun;YAN Song;TANG Shanshan;GU Haoyu(School of Environmental Science and Engineering,Changzhou University,Changzhou 213164,China;Institute of Eco-environmental and Soil Sciences,Guangdong Academy of Sciences,Guangzhou 510650,China;Rice National Engineering Laboratory(Nanchang),Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China)

机构地区：[1]常州大学环境科学与工程学院,江苏常州213164 [2]广东省科学院生态环境与土壤研究所,广州510650 [3]江西省农业科学院江西省超级水稻发展中心,南昌330200

出　　处：《农业环境科学学报》2025年第1期59-72,共14页Journal of Agro-Environment Science

基　　金：国家自然科学基金项目(41977138)。

摘　　要：为了阐明水稻不同生育期根际土溶磷菌对磷生物有效性的影响,选取了4个水稻品种:黄华占、桂野丰、中科早发粳1号和Sasanishiki,并分别在扬花期与成熟期收集了根际土壤样本。运用高通量测序技术分析了根际土溶磷菌群落的结构,对phoD基因进行研究,并探究了根际土溶磷菌群落与土壤理化性质及磷素组分的相关性。结果表明:假单胞菌科(Pseudomonadaceae,平均相对丰度为23.52%±31.03%)和硝化杆菌科(Nitrobacteraceae,平均相对丰度为11.69%±5.07%)是水稻根际土中的主要溶磷菌。成熟期溶磷菌的多样性(Chao1指数为105.03±9.75,Shannon指数为1.79±0.13)显著高于扬花期(Chao1指数为99.23±19.43,Shannon指数为1.50±0.55)。土壤pH值、速效磷含量和总氮含量是影响溶磷菌群落结构的关键环境因素。盐单胞菌科(Halomonadaceae)、叶杆菌科(Phyllobacteriaceae)和伯克氏菌科(Burkholderiaceae)的相对丰度与多种磷素组分有显著相关性,表明它们在水稻根际土壤的磷循环中具有重要作用。To assess the effects of rhizosphere soil bacteria on phosphorus availability at various rice growth stages,we studied four varieties:Huanghuazhan,Guiyefeng,Zhongkezaofajing No.1,and Sasanishiki.Soil samples from the rhizosphere were gathered at the flowering and maturity phases.We used high-throughput sequencing to examine the rhizosphere phosphate-solubilizing bacterial community,particularly the phoD gene.The study also investigated correlations with soil physicochemical characteristics and phosphorus fractions.The results showed that Pseudomonadaceae(23.52%±31.03%)and Nitrobacteraceae(11.69%±5.07%)dominated the rice rhizosphere as phosphorus solubilizing bacteria.Bacterial diversity at maturity,with Chao1 index of 105.03±9.75 and Shannon index of 1.79±0.13,was significantly greater compared to that flowering stage,which had Chao1 index of 99.23±19.43 and Shannon index of 1.50±0.55.Soil pH,available phosphorus,and total nitrogen were key factors affecting the phosphorus solubilizing bacterial community structure.Significant correlations between the relative abundances of Halomonadaceae,Phyllobacteriaceae,and Burkholderiaceae with different phosphorus fractions underscored their roles in the rice rhizosphere phosphorus cycle.

关 键 词：水稻 生育期 溶磷菌 phoD基因 Hedley磷分级 

分 类 号：S511[农业科学—作物学]