蚬壳花椒种子早期萌发过程中的分子机制  

Molecular mechanism of early germination stage of Zanthoxylum dissitum seeds

作　　者：李秋云 黄芳 孙吉康[1] 费玲瑜 王媛 安婷婷 曾艳玲[1] LI Qiuyun;HUANG Fang;SUN Jikang;FEI Lingyu;WANG Yuan;AN Tingting;ZENG Yanling(College of Life and Environmental Sciences,Central South University of Forestry&Technology,Changsha 410004,China)

机构地区：[1]中南林业科技大学生命科学与环境学院,长沙410004

出　　处：《植物生理学报》2024年第12期1769-1782,共14页Plant Physiology Journal

基　　金：湖南省重点研发计划(2022NK2006);湖南省自然科学基金(2021JJ31149);油料林农复合经营关键技术研究与示范项目(XLKY-202221);中南林业科技大学研究生科技创新基金(2024CX02072)。

摘　　要：蚬壳花椒是我国南方重要的珍稀木本药用植物,种子萌发周期长且自然萌发率低下。本研究用清水和赤霉素分别处理蚬壳花椒种子开展萌发试验,以期揭示其萌发机制。对萌发早期种子进行高通量转录组测序,基于转录组数据组装得到100982个UNIGENES,其中注释了59509个UNIGENES。共有42453个UNIGENES在种子萌发早期发生转录差异表达。蚬壳花椒种子后熟期长,其脱落酸(ABA)含量不利于种子的早期萌发。清水处理种子萌发早期开始抑制ABA合成、促进ABA分解,但降低ABA含量至利于种子持续萌发需要一定时间。种子萌发起始阶段淀粉和蔗糖代谢活跃,促使过氧化物如过氧化氢含量升高,但还原态抗氧化物如谷胱甘肽(GSH)含量较低,导致种子中丙二醛(MDA)含量升高,引发细胞过氧化损伤,使种子萌发失败。研究结果有助于了解蚬壳花椒种子早期难以萌发的分子机制。Zanthoxylum dissitum is an important woody medicinal plant in southern China,with a long germination period and low seed germination rate.The objective of this study was to study the germination of Z.dissitum seeds through the application of gibberellin(GA)and water.High-throughput transcriptome sequencing was performed on seeds at the early stage of germination.Based on transcriptome data,100982 UNIGENES were assembled based on transcriptome data,of which 59509 UNIGENES were annotated.A total of 42453 UNIGENES were differentially expressed during the early stage of seed germination.The post-ripening period of Z.dissitum seeds is long,and the ABA content of seeds is not conducive to the early germination.Although water treatment inhibited ABA synthesis and promoted ABA decomposition in the early stage of seed germination,it took a certain time to reduce ABA content to facilitate continuous seed germination.At the initial stage of seed germination,starch and sucrose metabolism were active,whic promoted the increase of peroxides such as hydrogen peroxide content,but the content of reduced antioxidants such as glutathione(GSH)was low,resulting in the increase of malondialdehyde(MDA)content in seeds.More MDA showed that more acute oxidative stress occurred,which caused the failure of seed germination.The results of this study are helpful to understand the molecular mechanism of the early germination of Z.dissitum seeds.

关 键 词：蚬壳花椒 种子萌发 赤霉素 转录组 氧化还原状态 

分 类 号：S573.9[农业科学—作物学]