Biofouling on titanium implants:a novel formulation of poloxamer and peroxide for in situ removal of pellicle and multi-species oral biofilm  

作　　者：Badra Hussain Roger Simm Jaime Bueno Savvas Giannettou Ali-Oddin Naemi Ståle Petter Lyngstadaas Håvard Jostein Haugen 

机构地区：[1]Department of Biomaterials,Institute of Clinical Dentistry,University of Oslo,Oslo,Norway [2]Institute of Oral Biology,University of Oslo,Oslo,Norway [3]Section of the Postgraduate program in Periodontology,Faculty of Dentistry,Complutense University,Madrid(UCM),Madrid,Spain

出　　处：《Regenerative Biomaterials》2024年第6期60-72,共13页再生生物材料(英文版)

基　　金：supported by the Research Council of Norway,‘MISFAITH’grant no.331752,‘Debrigel’grant no.332148 and Horizon 2020,BIOMATDB,grant no.101058779.

摘　　要：Eradicating biofouling from implant surfaces is essential in treating peri-implant infections,as it directly addresses the microbial source for infection and inflammation around dental implants.This controlled laboratory study examines the effectiveness of the four commercially available debridement solutions‘(EDTA(Prefgel^(■)),NaOCl(Perisolv^(■)),H_(2)O_(2)(Sigma-Aldrich)and Chlorhexidine(GUM^(■)Paroex^(■)))'in removing the acquired pellicle,preventing pellicle re-formation and removing of a multi-species oral biofilm growing on a titanium implant surface,and compare the results with the effect of a novel formulation of a peroxide-activated‘Poloxamer gel(Nubone^(■)Clean)'.Evaluation of pellicle removal and re-formation was conducted using scanning electron microscope(SEM),energy-dispersive X-ray spectroscopy and X-ray photoelectron spectroscopy to assess the surface morphology,elemental composition and chemical surface composition.Hydrophilicity was assessed through contact angle measurements.The multi-species biofilm model included Streptococcus oralis,Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans,reflecting the natural oral microbiome's complexity.Biofilm biomass was quantified using safranin staining,biofilm viability was evaluated using confocal laser scanning microscopy,and SEM was used for morphological analyses of the biofilm.Results indicated that while no single agent completely eradicated the biofilm,the‘Poloxamer gel'activated with‘H_(2)O_(2)'exhibited promising results.It minimized re-contamination of the pellicle by significantly lowering the contact angle,indicating enhanced hydrophilicity.This combination also showed a notable reduction in carbon contaminants,suggesting the effective removal of organic residues from the titanium surface,in addition to effectively reducing viable bacterial counts.In conclusion,the‘Poloxamer gel+H_(2)O_(2)'combination emerged as a promising chemical decontamination strategy for peri-implant diseases.It underlines the importanc

关 键 词：chemical decontamination cleaning of implant surfaces complex oral biofilms pellicle formation PERI-IMPLANTITIS 

分 类 号：TG1[金属学及工艺—金属学]