In situ co-deposition synthesis for collagen-Astragalus polysaccharide composite with intrafibrillar mineralization as potential biomimetic-bone repair materials  

作　　者：Han Li Ziying Guan Liren Wei Jian Lu Yanfei Tan Qingrong Wei 

机构地区：[1]National Engineering Research Center for Biomaterials(NERCB),Sichuan University,Chengdu 610065,P.R.China [2]College of Biomedical Engineering,Sichuan University,Chengdu 610065,P.R.China

出　　处：《Regenerative Biomaterials》2024年第6期125-136,共12页再生生物材料(英文版)

基　　金：supported by Sichuan Province Key Research and Development Project(grant number 2018SZ0046).

摘　　要：A hybrid material possessing both componential and structural imitation of bone tissue is the preferable composites for bone defect repair.Inspired by the microarchitecture of native bone,this work synthesized in vitro a functional mineralized collagen fibril(MCF)material by utilizing the method of in situ co-precipitation,which was designed to proceed in the presence of Astragalus polysaccharide(APS),thus achieving APS load within the biomineralized collagen-Astragalus polysaccharide(MCAPS)fibrils.Transmission electron microscope(TEM),selected area electron diffraction(SAED)and scanning electronic microscopy(SEM)identified the details of the intrafibrillar mineralization of the MCAPS fibrils,almost mimicking the secondary level of bone tissue microstructure.A relatively uniform and continuous mineral layer formed on and within all collagen fibrils and the mineral phase was identified as typical weak-crystalline hydroxyapatite(HA)with a Ca/P ratio of about 1.53.The proliferation of bone marrow-derived mesenchymal stem cells(BMSC)and mouse embryo osteoblast precursor cells(MC3T3-E1)obtained a significant promotion by MCAPS.As for the osteogenic properties of MCAPS,a distinct increase in the alkaline phosphatase(ALP)activity and the number of calcium nodules(CN)in BMSC and MC3T3-E1 was detected.The up-regulation of three osteogenic-related genes of RUNX-2,BMP-2 and OCN were confirmed via reverse transcription-quantitative polymerase chain reaction(RT-qPCR)to further verify the osteogenic performance promotion of MCAPS.A period of 14 days of culture demonstrated that MCAPS-L exhibited a preferable efficacy in enhancing ALP activity and CN quantity,as well as in promoting the expression of osteogenic-related genes over MCAPS-M and MCAPS-H,indicating that a lower dose of APS within the material of MCAPS is more appropriate for its osteogenesis promotion properties.

关 键 词：intrafibrillar mineralization collagen fibril CO-DEPOSITION Astragalus polysaccharide biomimetic-bone repair 

分 类 号：TB3[一般工业技术—材料科学与工程]