苹果NHX基因家族生物信息学及功能分析  

Bioinformatics and functional analysis of NHX gene family in apple

作　　者：朱红梅 王新亮 ZHU Hong-mei;WANG Xin-liang(Binzhou Public Utility Service Center,Binzhou,Shandong 256600,China;Shandong Key Laboratory of Eco-Environmental Science for the Yellow River Delta,Shandong University of Aeronautics,Binzhou,Shandong 256603,China)

机构地区：[1]滨州市公用事业服务中心,山东滨州256600 [2]山东航空学院/山东省黄河三角洲生态环境重点实验室,山东滨州256603

出　　处：《西南农业学报》2024年第12期2582-2591,共10页Southwest China Journal of Agricultural Sciences

基　　金：滨州学院博士学位人员及具有硕士学位的高级职称人员科研启动费项目(2019Y36)。

摘　　要：【目的】鉴定并分析苹果NHX基因家族的生物学信息、盐碱胁迫下的基因表达及其功能。【方法】利用氨基酸特异序列和关键词在苹果基因组中检索到17个NHX基因家族候选成员;通过Pfam数据库和SMART数据库最终确认10个苹果NHX基因家族成员,并分别命名为MdNHX1~MdNHX10;利用TBtools、MEGA-X、PlantCARE和MEME等软件对这10个苹果NHX基因家族成员做进一步分析,并利用RNA-seq测定MdNHX基因在盐碱胁迫下的表达情况。【结果】MdNHX基因编码蛋白质的氨基酸数为539~634 aa;分子量为59278.40~69769.84 Da;等电点为5.08~8.99,其中,只有MdNHX2和MdNHX4的等电点小于7,为酸性蛋白,其他成员的等电点均大于7,为碱性蛋白;不稳定系数为33.44~45.41,其中,MdNHX1、MdNHX4、MdNHX7和MdNHX8的不稳定系数小于40,属于稳定蛋白,其他成员均为不稳定蛋白;脂肪系数为101.48~115.52;平均亲水系数为0.364~0.583,均大于0,因此MdNHX可能均为疏水蛋白;它们具有10~11个跨膜螺旋结构,且均定位于液泡膜上;染色体定位和复制事件分析显示有4对MdNHX基因存在片段重复,这4对MdNHX同源基因的Ka/Ks值均小于1,表明苹果NHX基因家族进化过程存在较强的纯化选择;在盐碱胁迫下,多数MdNHX基因的表达被上调;蛋白相互作用分析显示,MdNHX蛋白主要与1-吡咯啉-5-羧酸合成酶、水孔蛋白、质膜ATP酶、乙醛脱氢酶等存在相互作用;GO功能分析表明MdNHX可能在调节苹果K^(+)和Na^(+)转运及盐碱胁迫响应中发挥重要作用。【结论】在苹果基因组GDDH13 v1.1中检索并鉴定得到10个完整的苹果NHX基因家族成员,进一步研究分析发现,苹果NHX基因家族成员可能在植物对胁迫、光以及植物激素的响应中发挥重要作用。[Objective]The present article aimed to identify and analyze the biological information of the apple NHX gene family,gene expression and function under salt alkali stress.[Method]Using amino acid specific sequences and keywords,17 candidate members of the NHX gene family were retrieved from the apple genome;Then,10 members of the apple NHX gene family were finally confirmed through the Pfam database and SMART database,and were named MdNHXI to MdNHXIO respectively;Finally,further analysis was conducted on these 10 members of the apple NHX gene family using software such as TBtools,MEGA-X,PlantCARE and MEME,and RNA-seq was used to determine the expression of the MdNHX gene under salt alkali stress.[Result]The number of amino acids encoded by these MdNHX genes was 539-634 aa;The molecular weight ranged from 59278.40 to 69769.84 Da;The isoelectric point was 5.08-8.99,among which only MdNHX2 and MdNHX4 had an isoelectric point less than 7,indicating acidic proteins,while the isoelectric points of other members were all greater than 7,indicating alkaline proteins;The instability coefficient ranged from 33.44 to 45.41,among which MdNHX1,MdNHX4,MdNHX7 and MdNHX8 had instability coefficients less than 40 and belonged to stable proteins,while the other members were all unstable proteins;The fat coefficient ranged from 101.48 to 115.52;The average hydrophilicity coefficient ranged from 0.364 to 0.583,all of which were greater than O,indicating that MdNHX may be hydrophobic proteins;They had 10-11 transmembrane spiral structures and were all located on the vacuolar membrane;Chromosome localization and replication event analysis showed that there were 4 pairs of MdNHX genes with fragment duplication,and the Ka/Ks values of these 4 pairs of MdNHX homologous genes were all less than 1,indicating strong purificationselection in the evolutionary process of the apple NHX gene family;Under salt alkali stress,the expression of most MdNHX genes was upregulated;Protein interaction analysis showed that MdNHX protein mainly interacted w

关 键 词：苹果 NHX基因家族 盐碱胁迫 基因表达 基因功能 

分 类 号：S661.1[农业科学—果树学]