二穗短柄草不同发育时期转录组分析  

Transcriptomic analysis of Brachypodium distachyon at different developmental stages

作　　者：杨龙姣 李亚娇 马培杰 陈才俊 刘晓霞 柳嘉佳 王小利 YANG Long-jiao;LI Ya-jiao;MA Pei-jie;CHEN Cai-jun;LIU Xiao-xia;LIU Jia-jia;WANG Xiao-li(Department of Grass Science,College of Animal Science,Guizhou University,Guiyang 550025,China;Guizhou Institute of Pratacultural,Guiyang 550006,China)

机构地区：[1]贵州大学动物科学学院草业科学系,贵阳550025 [2]贵州省草业研究所,贵阳550006

出　　处：《西南农业学报》2024年第12期2609-2619,共11页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(32060394);贵州省一般基金项目(黔科合基础-ZK[2023]一般164);贵州省科研机构创新能力建设专项(黔科合服企[2022]004);国家牧草产业技术(CARS-34);贵州省农业科学院2021年院基本科研业务发展专项(黔农科院青年科技基金[2021]31号)。

摘　　要：【目的】探究不同生长期二穗短柄草在光周期途径中的分子调控机制,为二穗短柄草种质资源保育、新品种选育与开发利用提供理论依据。【方法】以二穗短柄草植株幼苗期(YMQ)、分蘖期(FNQ)、孕穗期(YSQ)和抽穗期(CSQ)叶片为材料,采用转录组学测序技术对差异表达基因进行基因本体(Gene Ontology,GO)和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)功能富集分析,揭示植物二穗短柄草在长日照条件下的生理适应机制。【结果】检测获得12542个响应长日照胁迫的差异表达基因,其中,对比组合YMQ vs FNQ、FNQ vs YSQ和YSQ vs CSQ依次有5085个、2744个和4713个差异表达基因;GO富集发现差异表达基因显著富集在类囊体、核糖体亚基和细胞质核糖体等通路;KEGG富集发现差异基因显著富集在光合作用-天线蛋白和核糖体等通路;编码单核苷酸多态性(coding Single nucleotide polymorphism,c SNP)分析发现变异碱基总数为25880个,其中转换和颠换的碱基总数分别为18148个和7732个;4个时期共发现56个光周期途径基因,光周期差异表达基因主要富集在细胞质外体(RNase复合物)、光受体活性、核外体(RNase复合物)和昼夜节律-植物等通路;光周期途径关键基因GI、PRR1、PRR95、ELF4和COL3比FT、COL5、LHY、RUP1、HY5和COL9提前表达。【结论】在二穗短柄草不同发育时期中光周期关键基因表达差异显著,此结果可为进一步研究二穗短柄草开花的分子调控机理奠定基础。[Objective]The molecular regulatory mechanisms of Brachypodium distachyon at different growth stages in the photoperiod pathway were explored,which provided the theoretical basis for conserving germplasm resources,and breeding,developing and utilizing the new varie-ties of B.distachyon.[Method]Leaf samples at seedling stage(YMQ),illering stage(FNQ),booting stage(YSQ)and heading stage(CSQ)of B.distachyon were used as materials:Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)functional enrichment analysis of diferential expression genes were conducted by transcriptomic sequencing techniques,and finally the physiological ad-aptation mechanisms of B.distachyon under long-day conditions were revealed.[Result]A total of 12542 differential expression genes re-sponsive to long-day stress were identified.Among them,the comparative combinations of YMQ vs FNQ,FNQ vs YSQ and YSQ vs CSQ showed 5085,2744,and 4713 diferential expression genes,respectively.GO enrichment revealed that the differential expression genes were mainly enriched in pathways such as thylakoid,ribosomal subunit and cytosolic ribosome.KEGG enrichment revealed that differential genes were mainly enriched in pathways such as photosynthesis-antenna proteins and ribosome.The analysis of coding single nucleotide polymor-phisms(c SNP)revealed a total of 25880 variant bases,among which transitions and transversions were 18148 and 7732,respectively.A to-tal of 56 photoperiod pathway genes were identified at four stages,and photoperiod differential expression genes were mainly enriched in path-ways related to apoplast(RNase complex),photoreceptor activity,nuclear exosome(RNase complex)and circadian rhythm-plant.The photo-period pathway key genes such as GI,PRRI,PRR95,ELF4 and COL3 were expressed earlier than FT,COL5,LHY,RUPI,HY5 and COL9.[Conclusion]The photoperiod key genes during different developmental stages of B.distachyon expressed different significantly.And the results lay the foundation for studying the molecular regulatory mechanisms of

关 键 词：光周期 二穗短柄草 长日照 发育时期 转录组测序 

分 类 号：S519[农业科学—作物学] Q943.2[生物学—植物学]