外源赤霉素和多效唑诱导蓖麻伸缩茎节的转录组分析  

Transcription analysis of elongated and shortened stem node induced by exogenous gibberellin and paclobutrazol in Ricinus communis

作　　者：代梦媛 高梅[1] 胡尊红[1] 胡学礼[1] 杨谨[1] 李文昌[1] DAI Meng-yuan;GAO Mei;HU Zun-hong;HU Xue-li;YANG Jin;LI Wen-chang(Industrial Crops Institute of Yunnan Academy of Agricultural Sciences,Kunming 650205,China)

机构地区：[1]云南省农业科学院经济作物研究所,昆明650205

出　　处：《西南农业学报》2024年第12期2669-2682,共14页Southwest China Journal of Agricultural Sciences

基　　金：云南省科技厅建设面向南亚东南亚科技创新中心专项(202203AJ140002);昆明市国际(对外)科技合作基地项目(GHJD-2021018)。

摘　　要：【目的】旨在探索调控蓖麻株高生长的关键代谢通路及基因,以期为通过分子育种技术培育出理想株型的蓖麻新品种提供基因资源与理论依据。【方法】以外源赤霉素诱导蓖麻伸长茎节和多效唑诱导缩短茎节,通过转录组测序分析赤霉素处理后(GA vs CK)和多效唑处理后(PAC vs CK)的差异表达基因。【结果】赤霉素处理显著诱导1539个基因的差异表达,其中619个基因上调,920个基因下调;而多效唑处理则导致786个基因的差异表达,包括380个上调基因和406个下调基因。GO富集分析表明,这些差异表达基因主要富集于细胞组分构建、生物合成过程、信号传导及转录调控等关键生物学过程。特别值得注意的是,赤霉素氧化酶基因RcGA2ox7、RcGA2ox3在赤霉素处理下显著上调,而RcGA20ox3和RcGA20ox4则在多效唑处理下上调,这些基因通过调控体内活性赤霉素浓度,响应外源赤霉素和多效唑作用。KEGG富集分析结果显示,两组差异表达基因的主要富集通路包括植物激素信号传导途径、淀粉和蔗糖代谢途径以及苯丙烷类生物合成途径。在植物激素信号传导途径中,生长素、细胞分裂素、赤霉素、脱落酸、乙烯等多种激素相关基因均表现出显著的差异表达。在苯丙烷类生物合成途径中,催化单木质醇聚合为大分子木质素的过氧化物酶基因28320.t000063和30015.t000008响应赤霉素处理下调表达,响应多效唑处理上调表达。GA处理导致类黄酮合成途径的主要基因上调表达,PAC处理与之相反,GA处理促进类黄酮生物合成增加,导致木质素合成减少。【结论】通过转录组测序分析,成功筛选出一批可能参与蓖麻株高调控的关键基因,包括赤霉素氧化酶基因、生长素信号传导基因、木质素合成过程中的过氧化物酶基因以及类黄酮合成基因。这些发现不仅为揭示蓖麻株高调控的分子机制提供重要依据,也为未来通[Objective]The purpose of the study was to explore the key metabolic pathway and genes that regulated the castor plant height,in order to provide genetic resources and theoretical basis for breeding new varieties of ideotype by molecular breeding technology.[Method]The exogenous gibberellin(GA)inducing castor elongate stems and paclobutrazol(PAC)inducing curtate stems were used for RNA-seq and the differentially expressed genes(DEGs)between GA vs CK and PAC vs CK were analyzed respectively.[Result]Our analysis yielded 1539 significant DEGs in the GA vs CK comparison,comprising 619 upregulated and 920 downregulated genes.Similarly,the PAC vs CK comparison identified 786 significant DEGs,with 380 upregulated and 406 downregulated genes.Gene Ontology(GO)enrichment analysis underscored the significant enrichment of these DEGs in pathways related to cellular component organization and biogenesis,signal transducer activity,and transcription factor activity.Furthermore,we observed that GA treatment upregulated the expression of gibberellin oxidase genes RcGA2ox7 and RcGA2ox3,whereas PAC treatment upregulated RcGA20ox3 and RcGA20ox4.These four gibberellin oxidase genes played a crucial role in modulating the concentration of endogenously active gibberellins in response to exogenous GA and PAC treatments.In a more comprehensive analysis using KEGG pathways,we discovered that the DEGs were predominantly enriched in three major pathways:plant hor-mone signal transduction,starch and sucrose metabolism and phenylpropanoid biosynthesis.In the plant hormone signal transduction pathway,auxin,cytokinin,gibberellin,abscisic acid,ethylene and other hormone-related genes showed significant differential expression.In the phenyl-propanoid biosynthesis pathway,the expression of two peroxidase genes catalyzed the polymerization of monolith alcohols to macromolecular lignin was downregulated with GA treatment and upregulated with PAC treatment.In the flavonoid biosynthesis pathway,the expression of main genes was upregulated with GA trea

关 键 词：蓖麻 株高 转录组分析 激素信号传导 苯丙烷类生物合成 

分 类 号：S565.6[农业科学—作物学]