爪哇虫草菌与巴氏新小绥螨组合防控二斑叶螨技术初探  

Initial Exploration of Cordyceps javanica and Neoseiulus barkeri Combined Control Technology for Tetranychus urticae

作　　者：赵钰[1] 毛瑞霞 邢培翔 刁红亮 李先伟[1] 马瑞燕[1] ZHAO Yu;MAO Ruixia;XING Peixiang;DIAO Hongliang;LI Xianwei;MA Ruiyan(College of Plant Protection,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学植物保护学院,太谷030801

出　　处：《中国生物防治学报》2024年第6期1250-1259,共10页Chinese Journal of Biological Control

基　　金：省部共建有机旱作国家重点实验室(筹)(20220401091002-3-1);大同市·山西农业大学市校合作科研项目(DT-YXHZ-202102);晋中国家农高区植物保护教授、博士工作站(JZNGQBSGZZ001)。

摘　　要：为明确生防真菌爪哇虫草菌IJ-tg19联合巴氏新小绥螨防治二斑叶螨的应用潜力,在室内测定了菌株IJ-tg19对二斑叶螨和巴氏新小绥螨的致病力,并开展了菌+捕食螨防控温室二斑叶螨的盆栽和田间试验。结果显示:在浓度为1×10^(8)个孢子/mL孢子悬浮液处理下,菌株IJ-tg19对二斑叶螨成螨的致死中时LT50为3.23 d,接种后5 d致死中浓度LC_(50)为1.67×10^(5)个孢子/mL;两种生防措施无论单独应用还是联合应用,均可控制害螨虫口,但联合防治防效更高(96.74%、98.90%);“爪哇虫草菌-捕食螨”的田间应用试验中,对照区和处理区的二斑叶螨虫口密度存在显著性差异,对照区早于处理区24 d监测到二斑叶螨,对照区和处理区虫口密度峰值分别为102头/叶(11月29日)、7.3头/叶(12月5日)。本研究表明联合应用爪哇虫草菌IJ-tg19和巴氏新小绥螨防控温室二斑叶螨相比于两者单独应用效果更好,明确了菌+捕食螨防控温室二斑叶螨的生防应用潜力。To assess the potential applications of the Cordyceps javanica IJ-tg19 combined with Neoseiulus barkeri for the control of the Tetranychus urticae,the virulence of strain IJ-tg19 against T.urticae and the N.barkeri was determined in indoor experiments.Subsequently,greenhouse and field trials were conducted to evaluate the efficacy of the fungus combined with N.barkeri in controlling T.urticae.The results showed that under the treatment of a spore suspension with a concentration of 1×10^(8) spores/mL,the strain IJ-tg19 had a half-lethal time(LT50)of 3.23 days for the adult stage of the T.urticae,and a lethal concentration(LC_(50))of 1.67×10^(5) spores/mL at 5 days after inoculation.Both biological control measures,whether applied individually or in combination,were effective in controlling the T.urticae,but the combined treatment had a higher control efficacy(96.74%,98.90%).In the field application trial of“C.javanica-N.barkeri”in the control and treatment areas,there was a significant difference in the population density of the T.urticae.The T.urticae was detected 24 days earlier in the control area compared to the treatment area.The peak population densities in the control area and treatment area were 102 mites/leaf(29,November)and 7.3 mites/leaf(5,December)respectively.This study demonstrates that C.javanica IJ-tg19 combined with N.barkeri is more effective than their individual application,which highlights the potential of the application of fungus and N.barkeri for the prevention and biological control of greenhouse T.urticae.

关 键 词：爪哇虫草菌 二斑叶螨 巴氏新小绥螨 田间试验 生物防治 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]