解淀粉芽胞杆菌PHODG36种子处理干粉剂的制备及其对棉花黄萎病防治效果  

Development of Powder for Dry Seed Treatment based on Bacillus amyloliquefaciens PHODG36 and Its Control Efficacy on Cotton Verticillium Wilt

作　　者：赵卫松[1] 郭庆港[1] 鹿秀云[1] 勾建军 赵鸣[3] 李社增[1] 马平[1] ZHAO Weisong;GUO Qinggang;LU Xiuyun;GOU Jianjun;ZHAO Ming;LI Shezeng;MA Ping(Plant Protection Institute,Hebei Academy of Agriculture and Forestry Sciences/Innovation Centre of Hebei Province/Key Laboratory of IPM on Crops in Northern Region of North China,Ministry of Agriculture and Rural Affairs,Baoding 071000,China;Hebei Plant Protection and Inspection Station,Shijiazhuang 050031,China;Institute of Industrial Crops,Shandong Academy of Agricultural Sciences,Jinan 250100,China)

机构地区：[1]河北省农林科学院植物保护研究所/河北省农业有害生物综合防治工程技术研究中心/农业农村部华北北部作物有害生物综合治理重点实验室,保定071000 [2]河北省植保植检总站,石家庄050031 [3]山东省农业科学院经济作物研究所,济南250100

出　　处：《中国生物防治学报》2024年第6期1310-1318,共9页Chinese Journal of Biological Control

基　　金：河北省省级科技计划资助(22326509D);国家现代农业产业技术体系(CARS-15-19);国家重点研发计划(2022YFD1400300);河北省自然科学基金(C2022301036)。

摘　　要：微生物菌剂与植物残体联合施用能够有效降低植物病害发生。为进一步开发西兰花残体在防治棉花土传病害中的应用潜力,本研究以西兰花残体干粉为载体、解淀粉芽胞杆菌PHODG36为有效成分研制防治棉花黄萎病的种子处理干粉剂。通过对载体、助剂和母药比例的优化,明确100亿CFU/g解淀粉芽胞杆菌种子处理干粉剂的最佳配方为:20%菌株PHODG36母药、77%载体西兰花残体干粉、1%分散剂亚甲基双萘磺酸钠和2%润湿剂十二烷基苯磺酸钠。该制剂外观为浅黄色粉末,无团块,平均粒径D_(50)为37.65μm,pH为7.50,(54±2)℃贮藏14 d后未出现结块和发粘现象。温室试验结果表明,该菌剂对棉花株高、植株生物量表现促生作用,且对棉花黄萎病防效达73.03%。同时,田间小区试验表明其对棉花黄萎病防效达69.46%。河北和山东7地区试验示范结果表明,该种子处理剂对棉花黄萎病的防治效果为61.08%~74.8%,单株结铃增加3.38%~27.42%。综合分析表明,该菌剂能够有效防治棉花黄萎病发生,且提高单株结铃数量。因此,该菌剂在我国黄河流域棉花生产中具有广阔的应用前景。The combination application of microbial agents and plant residues could effectively reduce the incidence of plant diseases.In order to further develop the application potential of broccoli residues in the control of soil-borne diseases,a powder for dry seed treatment formulation(DS)for cotton Verticillium wilt was developed based on Bacillus amyloliquefaciens PHODG36 as the active ingredient,and dry broccoli residues powder as the carrier in the study.By optimizing the ratio of carrier,additives,and technical concentrate(TK),the optimal formula of 10 billion CFU/g B.amyloliquefaciens DS was established as follows:20%strain PHODG36 TK,77%broccoli residues dry powder,1%dispersant sodium salt of polynaphthalene sulphonic acid(NNO),and 2%wetting agent sodium dodecyl benzene sulfonate(SDBS).The results showed that PHODG36 DS had a light yellow appearance and no agglomeration,with an average particle size of 37.65μm,and there was no caking and sticking after 14 days of storage at(54±2)℃.The results showed that 10 billion CFU/g PHODG36 DS had a promoting effect on cotton plant height and biomass,and the control efficacy on CVW reached 73.03%in greenhouse experiments,61.60%in field plot experiments.Field trial demonstrations in different 7 regions(in Hebei and Shandong provinces)showed that the control efficacy on CVW ranged from 61.08%to 74.8%,and the number of bolls per plant increased from 3.38%to 27.42%.Comprehensive analysis showed that 10 billion CFU/g PHODG36 DS can effectively reduce the incidence of CVW and increase the number of bolls per plant.Therefore,it had broad application prospects for cotton production in the Yellow River Basin cotton region of China.

关 键 词：西兰花残体干粉 解淀粉芽胞杆菌 种子处理 棉花黄萎病 微生物菌剂 

分 类 号：S476[农业科学—农业昆虫与害虫防治]