拮抗菌与中药提取物复配对松枯梢病的抑菌效果筛选  

Screening of the Inhibitory Effect of Antagonistic Bacteria Compounded with Herbal Extracts on Sphaeropsis sapinea

作　　者：牛安琪 周德明[1,2,3,4] 缪新宇 陈行钢 周国英 刘君昂[1,2,3,4] NIU Anqi;ZHOU Deming;MIAO Xinyu;CHEN Xinggang;ZHOU Guoying;LIU Junang(Central South University of Forestry and Technology,Changsha 410004,China;Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests,Changsha 410004,China;Key Laboratory of National Forestry and Grassland Administration on Control of Artiffcial Forest Diseases and Pests in South China,Changsha 410004,China;Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees,Changsha 410004,China)

机构地区：[1]中南林业科技大学,长沙410004 [2]森林有害生物防控湖南省重点实验室,长沙410004 [3]南方人工林病虫害防控国家林业和草原局重点实验室,长沙410004 [4]经济林培育与保护教育部重点实验室,长沙410004

出　　处：《中国生物防治学报》2024年第6期1355-1365,共11页Chinese Journal of Biological Control

基　　金：国家重点研发计划课题(2022YFD1401005)。

摘　　要：松枯梢病是由松球壳孢菌Sphaeropsis sapinea引发的世界性松树病害,目前的防治手段主要集中于化学防治和生物防治。生物防治因取材于自然,具有绿色环保的先天优势而备受青睐。以筛选高效拮抗微生物为目的,本研究从马尾松根际土壤中筛选出一株对马尾松枯梢病有良好抑菌效果的解淀粉芽胞杆菌CSUFT-CSBA69,其抑制率为76.18%;以筛选潜在植物源药剂为目的,本研究对中药粗提物并进行毒力测定。结果表明黄连粗提物抑菌效果最佳,其EC_(50)值为0.033 mg/mL,最小抑菌浓度0.3125 mg/mL,最小杀菌浓度为0.625 mg/mL,浓度降至0.0097 mg/mL时仍有抑菌效果。为解决生产实践中单一生防因子效果不稳定的现实问题以及满足降低生防药剂成本的需求,本研究采用复配的方法探究菌株CSUFT-CSBA69与黄连低浓度提取液对S.sapinea的协同防治作用,其中黄连提取液终浓度为0.05 mg/mL、无菌滤液接种量为10%的复配液的抑菌效果为97.45%。研究说明,黄连提取物对病原菌有良好的抑菌效果,且能与生防菌复配,本项研究为复配制备生物源农药提供了新思路。Pine wilt is a worldwide pine disease caused by Sphaeropsis sapinea,The main control methods are chemical control and biological control.Biological control is favored because it is based on nature and has the inherent advantages of green environmental protection.In order to screen effective antagonistic microorganisms,a strain of Bacillus amyloliquefaciens CSUFT-CSBA69 was selected from rhizosphere soil of Pinus massoniana.The inhibition rate was 76.18%.The crude extracts of traditional Chinese medicine were tested and their toxicity was determined.The results showed that Coptis Chinensis had the best inhibitory effect.The EC_(50) value was 0.033 mg/mL,the MIC was 0.3125 mg/mL,the MBC was 0.625 mg/mL,and the bacteriostatic effect was still present when the concentration decreased to 0.0097 mg/mL.In order to solve the realistic problem of the unstable effect of a single biocontrol factor in production practice and meet the demand of reducing the cost of biocontrol agents,this study adopted the compound method to explore the synergistic control effect of strain CSUFT-CSBA69 and Coptis chinensis low-concentration extract on S.sapinea.The bacterial inhibition was 97.45%when the Coptis chinensis was 0.05mg/mL and the inoculated amount of aseptic filtrate was 10%.The study demonstrated that the extract of Coptis Chinensis has a good inhibitory effect on pathogenic bacteria and could be combined with biocontrol bacteria.This study provided a new idea for the preparation of bio-derived pesticides.

关 键 词：松枯梢病 解淀粉芽胞杆菌 植物提取物 复配 生物防治 

分 类 号：S476[农业科学—农业昆虫与害虫防治]