比较转录组分析揭示贝莱斯芽胞杆菌激活JA和SA途径增强花魔芋对软腐病抗性  

Comparative Transcriptomic Profiles Revealed JA-and SA-Regulated Soft Rot Disease Resistance by Bacillus velezensis BCP6 in Amorphophallus konjac

作　　者：张萌 陈森 唐登国 黄艳[1] 龙治坚[1] 王博雅 胡尚连[1] 曹颖[1] ZHANG Meng;CHEN Sen;TANG Dengguo;HUANG Yan;LONG Zhijian;WANG Boya;HU Shanglian;CAO Ying(School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621010,China)

机构地区：[1]西南科技大学生命科学与工程学院,绵阳621010

出　　处：《中国生物防治学报》2024年第6期1375-1385,共11页Chinese Journal of Biological Control

基　　金：国家现代农业产业技术体系四川创新团队项目(SC-CXTD-2024-12);四川省自然科学基金(24NSFSC1318);西南科技大学博士基金(23zx7145)。

摘　　要：贝莱斯芽胞杆菌Bacillus velezensis施用能够显著地提高花魔芋软腐病抗性,但具体机制并不清楚。本研究通过比较转录组分析探讨了贝莱斯芽胞杆菌BCP6对花魔芋应对软腐病菌P.aroidearum MY11侵染的响应和防御机制。差异表达分析、基因集富集分析(GSEA)及基因集变异分析(GSVA)显示贝莱斯芽胞杆菌BCP6上调了茉莉酸(jasmonic acid,JA)、活性氧以及细胞壁合成等相关通路,启动了魔芋基础防御体系;这种免疫激活使其在面对软腐病侵染时快速启动防御反应,增强了JA介导的诱导性系统抗性(ISR)和水杨酸(salicylic acid,SA)依赖的系统获得性抗性(SAR)信号通路,提高了细胞壁形成相关和病程相关蛋白基因的表达,有效提高了软腐病抗性。外源JA处理直接激活了细胞壁合成相关基因CESA2和CSI1的表达,而防御基因TLP和E13C显著被SA诱导。本研究结果为花魔芋软腐病生物防治和抗病育种奠定基础。The application of Bacillus velezensis has been proven to significantly enhance the resistance to soft rot in Amorphophallus konjac,yet its precise biological control mechanisms remain unclear.This study explored the effects of B.velezensis BCP6 on the defense pathways of A.konjac in response to P.aroidearum MY11 infection through comparative transcriptome analysis.Differential expression analysis,gene set enrichment analysis(GSEA)and gene set variation analysis(GSVA)revealed that BCP6 application upregulated pathways related to jasmonic acid(JA),reactive oxygen species,and cell wall synthesis,thereby activating the basic defense system of A.konjac.This immune activation facilitated the rapid initiation of defense responses when subjected to subsequent P.aroidearum infection by enhancing the JA-mediated induced systemic resistance(ISR)and SA-dependent systemic acquired resistance(SAR)signaling pathways.Simultaneously,a significant increase in the expression of genes involved in cell wall formation and pathogenesis-related proteins was observed,effectively enhancing resistance to soft rot.Meanwhile,exogenous JA treatment was found to directly stimulate the expression of cell wall-related genes,namely CESA2 and CSI1,while SA significantly induced the expression of SA-dependent defense genes TLP and E13C.The results provided a theoretical basis for biological control and disease-resistant breeding of konjac.

关 键 词：贝莱斯芽胞杆菌 软腐病抗性 转录组 诱导性系统抗性 系统获得性抗性 

分 类 号：S476[农业科学—农业昆虫与害虫防治]