强直性脊柱炎相关免疫球蛋白G N-糖基化标志物的筛选  

Screening of IgG N-glycosylation markers associated with ankylosing spondylitis

作　　者：温馨 尹嘉 周爱红 陶蕾 冉张申 罗文彦 刘淑琦 丁国永 宋代钰 Wen Xin;Yin Jia;Zhou Aihong;Tao Lei;Ran Zhangshen;Luo Wenyan;Liu Shuqi;Ding Guoyong;Song Daiyu(Department of Epidemiology,School of Public Health,Shandong First Medical University,Jinan 270117,China;Department of Rheumatology and Immunology,the Second Affiliated Hospital of Shandong First Medical University,Tai′an 271000,China;Cadre Health Care and Physical Examination Center,the Second Affiliated Hospital of Shandong First Medical University,Tai′an 271000,China;Experimental Teaching Center,School of Public Health,Shandong First Medical University,Jinan 270117,China)

机构地区：[1]山东第一医科大学公共卫生与健康管理学院流行病学研究室,济南270117 [2]山东第一医科大学第二附属医院风湿免疫科,泰安271000 [3]山东第一医科大学第二附属医院干部保健与查体中心,泰安271000 [4]山东第一医科大学公共卫生与健康管理学院实验教学中心,济南270117

出　　处：《中华风湿病学杂志》2025年第1期25-30,I0004,共7页Chinese Journal of Rheumatology

基　　金：山东省自然科学基金(ZR2020MH338);山东省大学生创新创业训练计划项目(S202210439034);山东第一医科大学公共卫生与健康管理学院学术提升计划(GW202218)。

摘　　要：目的对比分析强直性脊柱炎(AS)患者与健康人员的免疫球蛋白(Ig)G N-糖基标志物,探索IgG N-糖基作为AS潜在诊断标志物的能力。方法采用1∶1匹配病例-对照研究设计,招募2020年7月至2021年6月在泰安市中心医院和山东第一医科大学第二附属医院风湿免疫科就诊的81例AS患者,并配对81名健康体检对照。使用超高效液相色谱法定量检测人体血浆中IgG N-糖基化水平。采用二元logistic回归分析筛选AS相关的IgG N-糖基生物标志物。结果共有14个IgG N-糖基化直接指标和13个衍生指标在AS病例组与对照组之间的水平差异有统计学意义。二元logistic回归显示,聚糖峰(GP)4、无半乳糖基化结构(G0)、岩藻糖基化结构(F)和岩藻糖基化的无半乳糖基化的游离寡聚糖结构(FG0)与AS呈正相关[OR值(95%CI)分别为1.12(1.01,1.42)、1.21(1.03,1.43)、1.48(1.08,2.03)和1.27(1.04,1.55),P值分别为0.036,0.022,0.039,0.020]。在诊断效力方面,单一糖基GP4的受试者工作特征曲线下面积(AUC)最大,AUC(95%CI)为0.751(0.677,0.826),而联合糖基指标(GP4+G0+F+FG0)的AUC达到0.768(0.697,0.840)。结论IgG N-糖基可作为AS的候选生物标志物,值得进行后续研究。ObjectiveTo evaluate the potential of IgG N-glycans as diagnostic biomarker for ankylosing spondylitis(AS)by comparing and analyzing the IgG N-glycan profiles with AS and healthy controls.MethodsA 1∶1 matched case-control study design was adopted,81 AS patients who visited the Department of Rheumatology and Immunology at Taian City Central Hospital and the Second Affiliated Hospital of Shandong First Medical University between July 2020 and June 2021 were recruited.These patients were matched with 81 healthy individuals undergoing routine physical checkup.The levels of IgG N-glycosylation in human plasma were quantitatively measured using ultrahigh-performance liquid chromatography.Binomial logistic regression analysis was performed to identify IgG N-glycan biomarkers associated with AS.ResultsA total of 14 primary glycans and 13 derived traits showed statistically significant differences between the AS case group and the control group.Binomial logistic regression analysis showed that glycan peak 4,agalactosylated glycans,fucosylated glycans,and fucosylated agalactosylated glycans were positively associated with AS[OR(95%CI)=1.12(1.01,1.42),1.21(1.03,1.43),1.48(1.08,2.03),and 1.27(1.04,1.55);P=0.036,0.022,0.039,0.020,respectively].In terms of diagnostic performance,the single glycan GP4 exhibited the largest area under the ROC curve,with an AUC(95%CI)0.751(0.677,0.826),while the combined glycan indicators(GP4+G0+F+FG0)achieved an AUC(95%CI)0.768(0.697,0.840).ConclusionIgG N-glycans have the potentials to serve as candidate biomarkers for AS,and warrants further investigation.

关 键 词：脊柱炎 强直性 免疫球蛋白G 糖基化 生物标记 

分 类 号：R593.23[医药卫生—内科学]