Common key ingredient concluded in Baitouweng decoction,Gegen Qinlian decoction and Shaoyao decoction prevents radiation enteritis by regulating gut microbial network  

作　　者：Tian-hao Liu Ying-yue Sheng Chen-yang Zhang Hang Zhang Xue Li Li-hua Yu Ling-ling Xu Lin Zhao Hong Wei Ya-hong Zhou Yu-zheng Xue 

机构地区：[1]Department of Gastroenterology,Affiliated Hospital of Jiangnan University,Wuxi 214000,China [2]Wuxi School of Medicine,Jiangnan University,Wuxi 214122,China [3]Institute of Integrated traditional Chinese and Western Medicine,Affiliated Hospital of Jiangnan University,Wuxi 214000,China [4]Yu-Yue Pathology Scientific Research Center,Chongqing 401329,China [5]College of Animal Science and Technology,College of Animal Medicine,Huazhong Agricultural University,Wuhan 430070,China [6]Department of gynaecology,Wuxi Hospital Affiliated to Nanjing University of Chinese Medicine,Wuxi 214072,China.

出　　处：《Traditional Medicine Research》2024年第5期1-14,共14页TMR传统医学研究

基　　金：supported by the National Natural Sciences Foundation of China(82305072,82305064);Natural Science Foundation of Jiangsu Province(BK20230184);Science and technology development project of Jiangsu Provincial Administration of Traditional Chinese Medicine(YB2020043);Wuxi Municipal Health Commission Scientific Research Fund Youth Project(Q202106,Q202225);The Taihu Lake Talent Project of Wuxi Science and Technology Bureau(K20221027);Top Talent Support Program for young and middle-aged people of Wuxi Health Committee(HB2023044,HB2023051);Doctoral talent startup fund of Affiliated Hospital of Jiangnan University.

摘　　要：Background:The gut microbiota is crucial in the process of radiation enteritis(RE).As recommended by Chinese expert consensus,three formulas such as Baitouweng decoction,Gegen Qinlian decoction,and Shaoyao decoction,are used to prevent the same type of RE.Methods:Herein,we aimed to investigate the common pharmaceutical ingredients and molecular mechanisms of the three formulas on RE from the perspective of gut microbiota through multi-bioinformatics analysis and mouse experiment.Results:Interestingly,the important active ingredient screened was quercetin in all three herbal formulas,and the dominant targets that participated in RE treatment by targeting gut microbiota regulation were IL10,STAT1,IL1B,and IL1A.The concentration of quercetin was measured in Gegen Qinlian decoction>Shaoyao decoction>Baitouweng decoction(P<0.001)using high performance liquid chromatography.Moreover,a total of 40 SPF C57/BL6J(male,12-14 weeks old)mice were assigned to perform the validation experiment with various quercetin intervention doses.Quercetin significantly protected against inflammatory response in radiation induced-mice.The microbial alterations in the gut caused by quercetin intervention in radiation-induced mice were also explained.We further confirmed the alterations in the relative mRNA expression of the three formulas for the treatment of RE,which have four similar gut microbiota-regulated targets(IL10,STAT1,IL1B,and IL1A).Conclusion:These findings show that quercetin regulated the gut microbiota for RE therapy,a connotation that works on the principle of“different treatments for the same disease”for RE.The present investigation provides methodological guidelines and protocols for the modern use and examination of traditional therapeutic concepts.

关 键 词：different treatments for the same disease radiation enteritis gut microbiota QUERCETIN 

分 类 号：R28[医药卫生—中药学]