Inhibition of quorum sensing in drug-resistant Staphylococcus epidermidis by flavaspidic acid BB  

作　　者：Xiao-Fan Xiao Xiao-Feng Chen Shi-Qian Zheng Rong-Rong Deng Zhi-Bin Shen 

机构地区：[1]School of Chinese Materia Medica,Guangdong Pharmaceutical University,Guangzhou 510006,China [2]Guangdong Botanical Beauty Care Biotechnology Co.,Ltd.,Guangzhou 510600,China

出　　处：《Traditional Medicine Research》2024年第5期33-40,共8页TMR传统医学研究

摘　　要：Background:To investigate the effect of flavaspidic acid BB(BB)on biofilms and quorum sensing-related genes of drug-resistant Staphylococcus epidermidis(SE)and to provide a theoretical basis for the development of BB as a new type of quorum sensing inhibitor.Methods:The microdilution method was applied to screen 15 clinical isolates of S.epidermidis for drug-resistant S.epidermidis to be used as the test strain.The effects of BB on the biofilms of drug-resistant S.epidermidis at different growth stages were studied using the cell counting kit-8 assay and scanning electron microscopy.The gene expression of sigB,sarA,and luxS,regulators involved in quorum sensing of biofilm formation,was measured by PCR.Results:The minimum inhibitory concentrations of BB against 15 clinical strains of S.epidermidis ranged 11.67-66.67μg/mL.BB significantly inhibited biofilm formation by drug-resistant SE06 in the aggregation and maturation stages,with an activity superior to those of mupirocin and fusidic acid(P<0.05).At 40μg/mL,BB reduced the secretion of extracellular polymers and promoted the disruption of biofilm structure.At 80μg/mL,BB specifically interfered with the quorum sensing system of drug-resistant SE06 by downregulating sarA and luxS,thereby inhibiting this microbe’s biofilm formation.Conclusion:BB exhibited strong inhibitory activity against SE06 by suppressing biofilm formation and downregulating quorum sensing-related genes and was identified as a new potential quorum sensing inhibitor against S.epidermidis.

关 键 词：Dryopteris fragrans(L.)Schott flavaspidic acid BB Staphylococcus epidermidis BIOFILM quorum sensing 

分 类 号：R37[医药卫生—病原生物学]