Metabolic profiling and anti-inflammatory verification in the ethnic herbal medicine Trachelospermi Caulis et Folium  

作　　者：Zi-Hong Huang Chun-Hua Lai Bang-Yong Wang Bin-Sheng Luo Edward James Kennelly Qin-Gang Tan 

机构地区：[1]Department of Medicinal Chemistry,School of Pharmaceutical Sciences,Guilin Medical University,Guilin 541199,China [2]Department of Traditional Chinese Medicine,Affiliated Hospital of Guilin Medical University,Guilin 541001,China [3]Lushan Botanical Garden,Jiangxi Province and Chinese Academy of Sciences,Lushan 332900,China [4]Department of Biological Sciences,Lehman College,City University of New York,Bronx,New York 10468,USA [5]State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China.

出　　处：《Traditional Medicine Research》2024年第5期49-58,共10页TMR传统医学研究

基　　金：funded by National Natural Science Foundation of China(82060783);the Fund of State Key Laboratory of Phytochemistry and Plant Resources in West China,Chinese Academy of Sciences(P2022-KF13).

摘　　要：Background:Trachelospermi Caulis et Folium,the stems and leaves of Trachelospermum jasminoides(Lindl.)Lem,has been used as a remedy for rheumatism,gonarthritis,backache and pharyngitis,according to the Chinese Pharmacopoeia.However,the corresponding chemical substances of this ethnic medicinal plant with anti-inflammatory activity kept unclear.This study aimed to explore the active substances attribute to its anti-rheumatic activity of this medical plant.Methods:Ultra-high performance liquid chromatography combined with quadrupole time-of-flight mass spectrometry was used to identify the chemical components in the extract of T.jasminoides.Molecular docking was applied to analyze the binding affinity of potential active lignans to inducible nitric oxide synthase enzyme.The anti-inflammatory effects of the potential active fraction were verified by cell experiments in vitro.Results:Forty-one compounds,mainly are lignans and nine of which were reported for the first time from the genus of Trachelospermum,were characterized using ultra-high performance liquid chromatography quadrupole-time-of-flight mass spectrometry method.Molecular docking results showed these identified lignans had excellent interactions with the inducible nitric oxide synthase enzyme,and the lignans-enriched fraction could significantly inhibit the release of nitric oxide and the expression of inducible nitric oxide synthase protein in LPS-induced RAW264.7 cells.Conclusion:Lignans are the main active substances that responsible for the anti-inflammatory activity of the ethnic herbal medicine T.jasminoides.

关 键 词：Trachelospermi Caulis et Folium UPLC-QTOF-MS molecular docking NO inhibitory effect lignans 

分 类 号：R49[医药卫生—康复医学]