Rapid and Selective Detection of Foodborne Pathogens Using a Disposable Bio-sensing System Designed by Stepwise Antibody Immobilization on AuNPs@Cu-MOF Nanocomposite  

作　　者：Hend S.Magar Bahaa A.Hemdan Huda R.M.Rashdan Rabeay Y.A.Hassan 

机构地区：[1]Applied Organic Chemistry Department,National Research Centre(NRC),Dokki,Giza,12622,Egypt [2]Water Pollution Research Department,National Research Centre(NRC),Dokki,Giza,12622,Egypt [3]Chemistry of Natural and Microbial Products Department,Pharmaceutical and Drug Industries Research Institute,National Research Centre(NRC),Dokki,Giza,12622,Egypt [4]Biosensors Research Lab,University of Science and Technology(UST),Zewail City of Science and Technology,6 October City,Giza,12578,Egypt

出　　处：《Journal of Analysis and Testing》2024年第4期478-492,共15页分析检测(英文)

基　　金：Funding Authority(STDF)for granting the research project(STDF-ID:33682)。

摘　　要：There is an urgent need to provide a rapid and selective method to analyze food and water samples contaminated with Staphylococcus aureus since it is one of the most potential food and waterborne pathogens.Thus,a portable bio-sensing system and disposable bacterial-sensor chips consisted of anti-S.aureus@MPA/Au NPs-Cu-MOF@SPE were designed,fabricated,and applied for 20-min-based analysis of food and water samples.In this regard,screen-printed electrodes(SPEs)were functionalized with a new synthesized copper-metal organic framework(Cu-MOF)conjugated with gold nanoparticles(Au NPs)to eff ectively promote the chemical immobilization of the bio-receptor(anti-S.aureus antibody)onto the sensor matrix using the mercaptopropionic acid(MPA)as a chemical cross-linker.Subsequently,parameters aff ecting the bacterial-sensing effi-cacy were studied and optimized.Accordingly,high sensitivity was obtained with a wide dynamic linear range from 1×10 to 1×10^(7)colony forming units per milliliter(CFU/m L)with correlation coefficient(R^(2))of 0.990,limit of detection and limit of quantification of 0.132 CFU/m L,and 0.440 CFU/m L,respectively.High selectivity was provided since no electrochemical signals were obtained from various non-targeting strains of common pathogens,including the Pseudomonas aeruginosa,Escherichia coli,Salmonella enterica,Streptococcus mutans,and Enterococcus faecalis.Eventually,the newly developed handheld bacterial-sensing platform was applied for the rapid analysis of S.aureus in food and water samples.

关 键 词：Electrochemical biosensors Self-assembled monolayer IMMUNOSENSORS NANOSTRUCTURES Food Analysis Bacterial pathogens 

分 类 号：O657.1[理学—分析化学] TS207.4[理学—化学] R123.1[轻工技术与工程—食品科学]