微藻-亚硝化颗粒污泥对镉胁迫的响应研究  

Responses of microalgal-nitrifying granular sludge to cadmium stress

作　　者：陈召 刘世豪 朱丽娟 常德政 高岩[1,2] CHEN Zhao;LIU Shihao;ZHU Lijuan;CHANG Dezheng;GAO Yan(Henan Institute of Metallurgy Co.,Ltd.,Zhengzhou 450053,China;Research Center of Carbon Emission Reduction and Utilization Engineering Technology,Henan Academy of Sciences,Zhengzhou 450053,China)

机构地区：[1]河南省冶金研究所有限责任公司,河南郑州450053 [2]河南省科学院碳减排与利用工程技术研究中心,河南郑州450053

出　　处：《工业水处理》2025年第2期126-131,共6页Industrial Water Treatment

基　　金：河南省科学院基本科研业务费项目(220610050)。

摘　　要：为探究微藻-亚硝化颗粒污泥在镉胁迫下的响应,采用序批式光生物反应器对微藻-亚硝化颗粒污泥进行培养,向体系内添加不同浓度的Cd(Ⅱ)离子,对各阶段的污染物去除效果、胞外聚合物(EPS)、光合色素以及微生物种群特征的变化进行分析。结果表明,颗粒污泥对1 mg/L的Cd(Ⅱ)表现出较好的适应性,该阶段单位质量活性污泥中的EPS从46.2 mg/g增加至87.2 mg/g;5 mg/L的Cd(Ⅱ)对污泥产生一定程度的抑制;而10 mg/L的Cd(Ⅱ)会导致颗粒污泥体系的崩溃,Nitrosomonas的相对丰度在其胁迫下减少6.3%。微生物在低浓度Cd(Ⅱ)的刺激下大量分泌EPS,能够有效缓解其入侵,而高浓度的Cd(Ⅱ)可能会突破EPS的防御使微生物活性下降甚至死亡。Chlorella和Secenedesmus的相对丰度并未大幅波动,但光合色素的含量与Cd(Ⅱ)浓度呈负相关,Cd(Ⅱ)可能更多地影响藻类的光合能力,使体系内供氧不足进而影响亚硝化进程,但其并不会导致藻类大量死亡。To investigate the response of microalgae-nitrifying granular sludge to cadmium stress,a sequencing batch bioreactor was used to cultivate microalgae-nitrifying granular sludge.By adding different concentrations of Cd(Ⅱ)ions to the system,the pollutant removal efficiency,and changes of extracellular polymeric substances(EPS),photosynthetic pigments,and microbial community characteristics at each stage were analyzed.The results showed that granular sludge exhibited good adaptability to 1 mg/L Cd(Ⅱ).During this stage,the EPS in per unit mass of acti-vated sludge increased from 46.2 mg/g to 87.2 mg/g.Cd(Ⅱ)of 5 mg/L inhibited the sludge to a certain extent,while Cd(Ⅱ)of 10 mg/L led to the collapse of the granular sludge system.The relative abundance of Nitrosomonas de-creased by 6.3%under Cd(Ⅱ)stress.Microorganisms secreted a large amount of EPS under the stimulation of low concentrations of Cd(Ⅱ),which could effectively alleviate the invasion of Cd(Ⅱ),while high concentrations of Cd(Ⅱ)might break through the defense of EPS,leading to a decrease of microbial activity or even death.The rela-tive abundance of Chlorella and Secenedesmus did not fluctuate significantly,but the content of photosynthetic pig-ments was negatively correlated with Cd(Ⅱ)concentration.Cd(Ⅱ)might have a greater impact on the photosyn-thetic capacity of algae,leading to insufficient oxygen supply in the system and affecting the process of nitrification,rather than causing massive algal death.

关 键 词：重金属 亚硝化 颗粒污泥 胞外聚合物 光合色素 

分 类 号：X703[环境科学与工程—环境工程]