肺炎支原体肺炎患儿血清miR-509-5p和miR-222-3p表达水平与T淋巴细胞亚群的相关性研究  

A Study of the Correlation Between Serum MiR-509-5p and MiR-222-3p Expression Levels with T Lymphocyte Subsets in Children with Mycoplasma Pneumoniae Pneumonia

作　　者：单新洁 王龙 乌兰 黄倩文 SHAN Xinjie;WANG Long;Ulan;HUANG Qianwen(Department of Clinical Laboratory,Children's Hospital of Xinjiang Uygur Autonomous Region,Urumqi 83000,China)

机构地区：[1]新疆维吾尔自治区儿童医院检验科,新疆乌鲁木齐830000

出　　处：《标记免疫分析与临床》2025年第1期94-99,157,共7页Labeled Immunoassays and Clinical Medicine

摘　　要：目的 探究肺炎支原体肺炎患儿血清微小核糖核酸-509-5p(miR-509-5p)和微小核糖核酸-222-3p(miR-222-3p)表达水平与T淋巴细胞亚群的相关性。方法 取本院2023年2月至2024年2月收治的肺炎支原体肺炎患儿82例即为肺炎组,据其病情严重程度,分为轻度组(n=52)和重度组(n=30)。同期选择在本院体检健康的儿童82例为对照组。采用实时荧光定量PCR(qRT-PCR)对miR-509-5p和miR-222-3p水平进行检测。采用流式细胞术检测T淋巴细胞亚群(CD3^(+)、CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+))水平。Pearson相关性分析肺炎支原体肺炎患儿血清miR-509-5p和miR-222-3p表达水平与T淋巴细胞亚群的关系。分析血清miR-509-5p和miR-222-3p水平在诊断重度肺炎支原体肺炎患儿的价值。结果 肺炎组血清中miR-509-5p水平显著低于对照组(t=10.964,P<0.001),miR-222-3p水平显著高于对照组(t=12.832,P<0.001)。与对照组相比,肺炎组CD3^(+)、CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+)水平均显著降低(t=9.496、19.744、12.610、5.787,P<0.001)。Pearson相关性分析显示肺炎支原体肺炎患儿血清miR-509-5p水平与CD3^(+)(r_(P)=0.525,P<0.05)、CD4^(+)(r_(P)=0.623,P<0.05)、CD8^(+)(r_(P)=0.663,P<0.05)、CD4^(+)/CD8^(+)(r_(P)=0.741,P<0.05)水平呈正相关关系,miR-222-3p与CD3^(+)(r_(P)=-0.535,P<0.05)、CD4^(+)(r_(P)=-0.662,P<0.05)、CD8^(+)(r_(P)=-0.684,P<0.05)、CD4^(+)/CD8^(+)(r_(P)=-0.691,P<0.05)水平呈负相关关系。与轻度组相比,重度组患者血清中miR-509-5p水平显著降低(t=5.046,P<0.001),miR-222-3p水平显著升高(t=4.372,P<0.001)。ROC曲线结果显示,与miR-509-5p和miR-222-3p单独诊断相比,两者联合检测对重度肺炎支原体肺炎患儿诊断的AUC显著升高(ZmiR-509-5p~miR-509-5p+miR-222-3p=1.958,P=0.040;ZmiR-222-3p~miR-509-5p+miR-222-3p=1.878,P=0.042)。结论 肺炎支原体肺炎患儿血清miR-509-5p低表达,miR-222-3p高表达,二者与T淋巴细胞亚群有关,miR-509-5p和miR-222-3p检测对重度肺炎支原体肺炎患儿有�Objective To investigate the correlation between the expression levels of serum microribonuclease-509-5p(miR-509-5p) and microribonuclease-222-3p(miR-222-3p) and T lymphocyte subsets in children with Mycoplasma pneumoniae pneumonia, so as to evaluate the value of serum miR-509-5p and miR-222-3p levels in the diagnosis of severe Mycoplasma pneumoniae in children. Methods A total of 82 children with Mycoplasma pneumoniae pneumonia admitted to our hospital from February, 2023 to February, 2024 were included in the pneumonia group, which was further divided into a mild group(n=52) and a severe group(n=30) according to the severity of the disease. During the same period, 82 healthy children who underwent physical examinations at our hospital were included as the control group. Real-time fluorescence quantitative PCR(qRT-PCR) was applied to measure the levels of miR-509-5p and miR-222-3p. Flow cytometry was applied to detect the levels of T lymphocyte subsets(CD3^(+), CD4^(+), CD8^(+), CD4^(+)/CD8^(+)). Pearson correlation method was performed to identify the potential relationships between serum miR-509-5p and miR-222-3p expression levels with T lymphocyte subsets in children with Mycoplasma pneumoniae pneumonia. Results The serum miR-509-5p level in the pneumonia group was significantly lower than that in the control group(t=10.964, P<0.001), while the serum miR-222-3p level was significantly higher than that in the control group(t=12.832, P<0.001). Compared with the control group, the levels of CD3^(+), CD4^(+), CD8^(+), CD4^(+)/CD8^(+) in the pneumonia group were significantly decreased(t=9.496, 19.744, 12.610, 5.787, P<0.001). Pearson correlation analysis showed that serum miR-509-5p levels were positively correlated with CD3^(+)(r_P=0.525, P<0.05), CD4^(+)(r_P=0.623, P<0.05), CD8^(+)(r_P=0.663, P<0.05), CD4^(+)/CD8^(+)(r_P=0.741, P<0.05) levels, while miR-222-3p was negatively associated with CD3^(+)(r_P=-0.535, P<0.05), CD4^(+)(r_P=-0.662, P<0.05), CD8^(+)(r_P=-0.684, P<0.05), CD4^(+)/CD8^(+)(r_P=-0.691, P<0.05)

关 键 词：肺炎支原体肺炎 微小核糖核酸-509-5p 微小核糖核酸-222-3p T淋巴细胞亚群 相关性 

分 类 号：R725.6[医药卫生—儿科]