不同严重程度特发性少弱精子症患者血清Th1/Th2细胞因子水平变化  

Changes of serum Th1/Th2 cytokines in patients with different degrees of idiopathic oligonasthenospermia

作　　者：杨磊 冯美宁 宋海锋 许涛 Yang Lei;Feng Meining;Song Haifeng;Xu Tao(Department of Reproductive Medicine,Xianyang Central Hospital,Xianyang 712000,China;Department of Medical Laboratory,Xianyang Central Hospital,Xianyang 712000,China)

机构地区：[1]咸阳市中心医院生殖医学科,咸阳712000 [2]咸阳市中心医院医学检验科,咸阳712000

出　　处：《中国医师杂志》2025年第1期38-41,46,共5页Journal of Chinese Physician

基　　金：陕西省重点研发计划项目(2020SF-176)。

摘　　要：目的探究不同严重程度特发性少弱精子症(IO)患者血清辅助性T细胞1(Th1)/辅助性T细胞2(Th2)细胞因子水平变化。方法前瞻性选取2020年1月—2022年6月咸阳市中心医院收治的IO患者136例,根据IO病情严重程度分为轻中度组(86例)和重度组(50例);另选取同期体检健康的60例男性为正常对照组。比较三组基线资料、血清Th1和Th2细胞因子[干扰素-γ(IFN-γ)、肿瘤坏死因子-α(TNF-α)和白细胞介素(IL)-2、IL-4、IL-6、IL-10、IL-21],采用Spearman相关分析法评价IFN-γ、TNF-α、IL-2、IL-4、IL-6、IL-10、IL-21与IO病情严重程度的相关性。结果重度组精子浓度、精子总数、前向运动精子百分率低于轻中度组和正常对照组(均P<0.05),轻中度组精子浓度、精子总数、前向运动精子百分率低于正常对照组(均P<0.05)。重度组血清Th1细胞因子IFN-γ、TNF-α、IL-2、IL-21水平高于轻中度组和正常对照组(均P<0.05),轻中度组血清Th1细胞因子IFN-γ、TNF-α、IL-2、IL-21水平高于正常对照组(均P<0.05)。重度组血清Th2细胞因子IL-4、IL-6、IL-10水平高于轻中度组和正常对照组(均P<0.05),轻中度组血清Th2细胞因子IL-4、IL-6、IL-10水平高于正常对照组(均P<0.05)。Spearman相关分析结果显示,IO病情严重程度与血清Th1细胞因子(IFN-γ、TNF-α、IL-2、IL-21)和血清Th2细胞因子(IL-4、IL-6、IL-10)水平均呈正相关(均P<0.001)。结论IO患者的Th1/Th2免疫失衡随着疾病严重程度的增加而加重,临床应强化IO早期诊断并尽早治疗,预防疾病进展。Objective To investigate the changes of serum helper T cell 1(Th1)/helper T cell 2(Th2)cytokines in patients with idiopathic oligonasthenospermia(IO).MethodsA total of 136 patients with IO admitted to the Xianyang Central Hospital from January 2020 to June 2022 were prospectively selected and divided into mild to moderate group(86 cases)and severe group(50 cases)according to the severity of IO.Another 60 healthy males were selected as normal control group.Baseline data,serum Th1 and Th2 cytokines[interferon-γ(IFN-γ),tumor necrosis factor-α(TNF-α)and interleukin(IL)-2,IL-4,IL-6,IL-10,IL-21]of the three groups were compared.Spearman correlation analysis was used to evaluate the correlation between IFN-γ,TNF-α,IL-2,IL-4,IL-6,IL-10,IL-21 and the severity of IO disease.ResultsThe sperm concentration,total sperm and percentage of forward motile sperm in severe group were lower than those in mild to moderate group and normal control group(all P<0.05),and the sperm concentration,total sperm and percentage of forward motile sperm in mild to the moderate group were lower than those in the normal control group(all P<0.05).The serum levels of Th1 cytokines IFN-γ,TNF-α,IL-2 and IL-21 in severe group were higher than those in the mild-moderate group and the normal control group(all P<0.05),and the serum levels of Th1 cytokines IFN-γ,TNF-α,IL-2 and IL-21 in mild-moderate group were higher than those in the normal control group(all P<0.05).The serum levels of Th2 cytokines IL-4,IL-6 and IL-10 in severe group were higher than those in the mild-moderate group and the normal control group(all P<0.05),and the serum levels of Th2 cytokines IL-4,IL-6 and IL-10 in mild-moderate group were higher than those in the normal control group(all P<0.05).Spearman correlation analysis showed that the severity of IO disease was positively correlated with the levels of serum Th1 cytokines(IFN-γ,TNF-α,IL-2,IL-21)and serum Th2 cytokines(IL-4,IL-6,IL-10)(all P<0.001).ConclusionsThe Th1/Th2 immune imbalance in patients with IO is aggravate

关 键 词：特发性少弱精子症 TH1细胞 TH2细胞 

分 类 号：R698.2[医药卫生—泌尿科学]