基于网络药理学分析半枝莲治疗肺纤维化的作用机制  

Research on the mechanism of Scutellariae barbatae Herba in treating pulmonary fibrosis based on network pharmacology

作　　者：王杉宇 郑远江 赵曦 饶珊珊[3] WANG Shanyu;ZHENG Yuanjiang;ZHAO Xi;RAO Shanshan(The First Clinical Institute,Zunyi Medical University,Guizhou Province,Zunyi 563000,China;Department of Respiratory and Critical Care Medicine,Ziyang Central Hospital,Sichuan Province,Ziyang 641300,China;Department of Respiratory and Critical Care Medicine,Guizhou Provincial People's Hospital,Guizhou Province,Guiyang 550002,China)

机构地区：[1]遵义医科大学第一临床学院,贵州遵义563000 [2]四川省资阳市中心医院呼吸与危重症医学科,四川资阳641300 [3]贵州省人民医院呼吸与危重症医学科,贵州贵阳550002

出　　处：《中国当代医药》2025年第3期4-9,共6页China Modern Medicine

基　　金：贵州省科技计划项目(黔科合支撑[2021]一般054)。

摘　　要：目的以网络药理学为工具,筛选半枝莲有效成分作用靶点,明确其作用于肺纤维化的机制,进一步为肺纤维化的临床诊疗提供理论基础。方法在中药系统药理学数据库与分析平台(TCMSP)中检索半枝莲,下载其有效化学成分及其对应的作用靶点信息。在GeneCards数据库中搜索得到肺纤维化有关的靶点基因,筛选出二者重叠的靶点,利用Cytoscape软件对重复靶点进行分析,构建“成分-靶点-疾病”相互作用网络。将半枝莲治疗肺纤维化的可能靶点导入STRING数据库进行蛋白相互作用网络分析、筛选出关键靶点。在R studio中加载ClusterProfiler软件包,对筛选出的共同靶点进行GO及KEGG富集分析。结果共获得半枝莲29个活性化合物,作用于人体的不重复的靶点191个。半枝莲治疗肺纤维化的5个关键靶点为AKT1、IL-6、IL-1β、VEGFA、JUN。结论半枝莲治疗肺纤维化的药理机制可能与其协同调节氧化应激、脂质代谢、炎症反应、细胞凋亡、细胞周期、分化、血管生成密切相关。Objective Using network pharmacology as a tool,to screen the targets of the active components of Scutellariae barbatae Herba,clarify the mechanism of its action on pulmonary fibrosis,and further provide a theoretical basis for the clinical diagnosis and treatment of pulmonary fibrosis.Methods Scutellariae barbatae Herba was searched in Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),and its effective chemical constituents and their corresponding target information were downloaded.The target genes related to pulmonary fibrosis were searched in the GeneCards database,and the overlapping targets were selected.The repeated targets were analyzed by Cytoscape software,and the"component-target-disease"interaction network was constructed.Possible targets of Scutellariae barbatae Herba in the treatment of pulmonary fibrosis were imported into STRING database for protein interaction network analysis and key targets were screened.The ClusterProfiler package was loaded in R studio,and GO and KEGG enrichment analysis was performed on the selected common targets.Results A total of 29 active compounds of Scutellariae barbatae Herba were obtained,and 191 non-repetitive targets were obtained.The five key targets of Scutellariae barbatae Herba in the treatment of pulmonary fibrosis were AKT1,IL-6,IL-1β,VEGFA and JUN.Conclusion The pharmacological mechanism of Scutellariae barbatae Herba in treating pulmonary fibrosis may be closely related to its synergistic regulation of oxidative stress,lipid metabo-lism,inflammatory response,apoptosis,cell cycle,differentiation and angiog-enesis.

关 键 词：半枝莲 网络药理学 肺纤维化 靶基因 信号通路 

分 类 号：R563[医药卫生—呼吸系统]