3D bioprinting of a dermal scaffold for full-thickness skin tissue regeneration  

用于全层皮肤组织再生的真皮支架3D打印

作　　者：Lu Han Zixian Liu Meng Li Zhizhong Shen Jianming Wang Shengbo Sang 韩璐;刘姿娴;李梦;申治中;王建明;桑胜波(微纳传感与人工智能感知山西省重点实验室,太原市030024;太原理工大学新型传感器与智能控制教育部重点实验室,太原市030024;山西浙大新材料与化工研究院,太原市030024;山西省六维人工智能生物医学研究院,太原市030031;太钢总医院,太原市030809)

机构地区：[1]Shanxi Key Laboratory of Micro Nano Sensors&Artificial Intelligence Perception,College of Electronic Information and Optical Engineering,Taiyuan University of Technology,Taiyuan 030024,China [2]Key Laboratory of Advanced Transducers and Intelligent Control System of the Ministry of Education,Taiyuan University of Technology,Taiyuan 030024,China [3]Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering,Taiyuan 030024,China [4]Shanxi Research Institute of 6D Artificial Intelligence Biomedical Science,Taiyuan 030031,China [5]General Hospital of TISCO,Taiyuan 030809,China

出　　处：《Bio-Design and Manufacturing》2025年第1期68-84,I0039-I0041,共20页生物设计与制造(英文)

基　　金：supported by the National Natural Science Foundation of China(Nos.51975400 and 62031022);the Shanxi Provincial Key Medical Scientific Research Project(No.2020XM06);the Shanxi Provincial Basic Research Project(Nos.202103021221006,20210302123040,and 202103021223069);the Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(No.2021L044);the Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering(No.2022SX-TD026).

摘　　要：Dermal substitutes have provided a template for the regeneration and reconstruction of the dermis.However,the healed skin tissue often exhibits abnormal morphology and functionality,including scarring and inflammation.In this study,a composite bioink composed of methacrylated gelatin(GelMA)and chitosan oligosaccharide(COS)was proposed for printing a dermal scaffold using digital light processing(DLP)technology.The GelMA/COS bioink exhibited suitable porosity,swelling,degradation rate,and mechanical properties.The inclusion of COS demonstrated antibacterial effects against both Gram positive and Gram-negative bacteria,while simultaneously fostering the proliferation of human dermal fibroblasts(HDFs).Additionally,the application of COS could effectively reduce the expression levels of fibrosis-related genes,such as collagen I,collagen III,and fibronectin I.The three-dimensionally printed cell-laden dermal scaffold exhibited excellent shape fidelity and high cellular viability,facilitating the extension of HDFs along the scaffold and the simultaneous secretion of extracellular matrix proteins.Furthermore,the HDF-laden dermal scaffold transplanted into full-thickness skin defect sites in nude mice was shown to accelerate wound closure,reduce inflammation,and improve wound healing.Overall,the DLP-printed dermal scaffold provides an appealing approach for effectively treating full-thickness skin defects in clinical settings.真皮替代物为真皮的再生和重建提供了一个模板。然而,愈合后的皮肤组织往往表现出异常的形态和功能,包括疤痕和炎症。本研究提出一种由甲基丙烯酸明胶(GelMA)和壳寡糖(COS)组成的复合生物墨水,利用数字光处理(DLP)技术打印真皮支架。GelMA/COS生物墨水具有合适的孔隙率、溶胀率、降解率和力学性能。COS对革兰氏阳性和革兰氏阴性细菌均有抗菌作用,同时可促进人真皮成纤维细胞(HDFs)的增殖。此外,COS的应用可以有效降低胶原I、胶原III和纤维连接蛋白I等纤维化相关基因的表达水平。通过3D打印制备的真皮支架具有优异的形状保真度和高细胞活力,促进了HDFs沿支架的延伸,同时能够分泌细胞外基质相关的蛋白。将负载HDFs的真皮支架移植到裸鼠全层皮肤缺损部位,可加速创面愈合,减少炎症,提高伤口愈合质量。总的来说,DLP打印的真皮支架为临床环境中有效治疗全层皮肤缺陷提供了一种吸引人的方法。

关 键 词：3D printing Dermal scaffold PHOTO-CROSS-LINKING Skin tissue regeneration 

分 类 号：G63[文化科学—教育学]