Large-Scale Environmental DNA Metabarcoding Shows a Comprehensive Fish Community in the East China Sea:Do Water Layer and Habitat Matter?  

作　　者：WANG Xiaoyan ZHANG Haobo ZHONG Lanping SHIH Yijia JI Fenfen GAO Tianxiang 

机构地区：[1]National Engineering Research Center of Marine Facilities Aquaculture,Zhejiang Ocean University,Zhoushan 316004,China [2]Chinese Sturgeon Research Institute,China Three Gorges Corporation,Yichang 443100,China [3]Fisheries College,Jimei University,Xiamen 361021,China [4]Fishery College,Zhejiang Ocean University,Zhoushan 316004,China

出　　处：《Journal of Ocean University of China》2025年第1期217-228,共12页中国海洋大学学报(英文版)

基　　金：funded by the National Key Research and Development Program of China(No.2023YFD2401903);the National Natural Science Foundation of China(No.41806180);the Science and Technology Project of Zhoushan(No.2022C41010);the Scientific Research Project of China Three Gorges Corporation(No.WWKY-2020-0079);the Shared Voyage Program of the National Natural Science Foundation of China in the East China Sea(No.NORC2019-02)。

摘　　要：Biodiversity serves as the foundation for human survival and development.Marine fishes play a crucial role in supporting both global biodiversity and food sources.The conventional methods for fish diversity and fishery resources survey are inadequate to meet the growing demand for large-scale and long-period surveys.The eDNA metabarcoding approach provides an effective and noninvasive monitoring revolution,and has been applied to identify fish diversities.This study conducted a comprehensive comparison of fish diversity in the East China Sea on a large scale both horizontally and vertically.The results showed a total of 81 fish species,which were classified into 20 orders,44 families and 72 genera.Although no statistically significant difference in fish diversity was found amongst the horizontal groups(east-middle-west,south-middle-north,and shallow-middle-deep)and the various sampling layers(surface-middle-bottom),discernible trends were obtained in this study.Fish diversity results revealed the orders of east>middle>west,middle>north>south and deep>shallow>middle in horizontal groups.Additionally,surface water exhibited a higher level of fish diversity than other water layers.Some fish species were only detected at specific vertical depths.The diverse composition of fish species across different water layers can be attributed to their distinct ecological types.The overall findings suggest that an increase of sampling stations and mixed samples should be collected in deeper water bodies for comprehensive understanding of fish diversity.This study can also provide the fundamental data and experimental design reference for future studies and fishery management.

关 键 词：environmental DNA metabarcoding fish diversity spatial distribution East China Sea 

分 类 号：R73[医药卫生—肿瘤]