Strategies for efficient enrichment of anaerobic ammonia oxidizing bacteria in activated sludge  

作　　者：Bin Tang Jin Wang Xingdong Gao Zhihua Li 

机构地区：[1]Key Laboratory of Northwest Water Resource,Environment,and Ecology,MOE,School of Environmental and Municipal Engineering,Xi’an University of Architecture and Technology,Xi’an 710055,China [2]Xi’an Key Laboratory of Intelligent Equipment Technology for Environmental Engineering,Xi’an University of Architecture and Technology,Xi’an 710055,China

出　　处：《Journal of Environmental Sciences》2025年第5期703-713,共11页环境科学学报(英文版)

基　　金：supported by the National Natural Science Foundation of China(No.52070149);Shaanxi Innovative Research Team for Key Science and Technology(No.2023-CXTD-36);Shaanxi Province Key Program for International S&T Cooperation Projects(No.2024GH-ZDXM-04);the Bureau of Science and Technology of Xi’an City of China(No.23SFSF0011).

摘　　要：Anaerobic ammonia oxidation(Anammox)is an economical and sustainablewastewater nitrogen removal technology,and its application in the mainstream process is the inevitable trend of the development of Anammox.However,how to effectively enriching Anammox bacteria from the activated sludge remains challenging and restricts its extensive applications.In this study,the rapid and efficient enrichment of Anammox bacteriawas achieved by raising the reflux ratio and nitrogen loading rate(NLR)using conventional activated sludge as the inoculant.In the screening phase(days 1–90),the reflux ratio was increased to discharge partial floc sludge,resulting in the relative abundance of Candidatus Brocadiaceae increased from0.04%to 22.54%,which effectively reduced thematrix and spatial competition between other microorganisms and Anammox bacteria.On day 90,the stoichiometric ratio of the Anammox process closely approached the theoretical value of 1:1.32:0.26,indicating that the Anammox reaction was the primary nitrogen removal process in the system.In the enrichment phase(days 91–238),the NLR increased from 0.43 to 1.20 kgN/(m^(3)·d)and removal efficiency was 71.89%,resulting in the relative abundance of Candidatus Brocadiaceae increased to 61.27%on day 180.The reactor operated steadily from days 444 to 498,maintaining the nitrogen removal rate(NRR)of 3.00 kgN/(m^(3)·d)and achieving successful sludge granulation with the particle size of 392.4μm.In short,this study provided a simple and efficient approach for enriching Anammox bacteria from the activated sludge,supporting to start an Anammox process efficiently.

关 键 词：ANAMMOX Activated sludge ENRICHMENT Nitrogen removal Strategy 

分 类 号：X70[环境科学与工程—环境工程]