木姜叶柯引种后主要药用成分的稳定性及变异研究  

Study on stability and variation of major medicinal components after its introduction of Lithocarpus litseifolius

作　　者：羊奕珣 田朝霞 杨志玲[1] 杨旭[1] 谭梓峰[1] 赵鹏霞 YANG Yixun;TIAN Zhaoxia;YANG Zhiling;YANG Xu;TAN Zifeng;ZHAO Pengxia(Institute of Subtropical Forestry,Chinese Academy of Forestry,Hangzhou 311400,China;College of Landscape Architecture,Nanjing Forestry University,Nanjing 210037,China;College of Horticulture,Hebei Agricultural University,Baoding,Hebei 071000,China)

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江杭州311400 [2]南京林业大学风景园林学院,江苏南京210037 [3]河北农业大学园艺学院,河北保定071000

出　　处：《江西农业大学学报》2025年第1期117-129,共13页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金面上项目(32071785);中央级公益性科研院所基本科研业务费专项资金(CAFYBB2019SY016)。

摘　　要：【目的】木姜叶柯茶药历史悠久,嫩叶中含有丰富的黄酮类、根皮苷等成分,因其对于糖尿病等治疗疗效显著而备受关注。基于引种是扩大资源的重要方式,本研究拟利用多个统计方法分析木姜叶柯不同种源引种到多个异质园基地后主要药用成分的稳定性及变异特征,为其引种及资源利用奠定基础。【方法】将6个省区12个种源分别引种到3个异质园试验基地,测定药效成分总黄酮、三叶苷、根皮苷、二氢查耳酮、三叶苷/根皮苷比值,利用单因素方差分析(one-way ANOVA)、多重比较(Duncan’s)、主成分分析和聚类分析4种统计方法比较和分析引种前后主要药用成分的稳定性、差异、综合得分及聚类特点。【结果】发现引种后木姜叶柯的主要药效成分相似度为0.875~1.000,证实引种后主要药效成分相对较稳定。本试验共引种36次,研究获知总黄酮、三叶苷、根皮苷、二氢查耳酮、三叶苷/根皮苷比值等高于原产地时引种次数分别为21、10、27、14和5,占总次数的58.33%、27.78%、75.00%、38.89%和13.89%。聚类分析发现在欧氏距离为5时36份试验材料聚成4类,其中第3类包括11份,其总黄酮、三叶苷、根皮苷和二氢查耳酮含量均较高,大多高于原产地。主成分分析发现浙江江山、湖南溆浦、广东惠州、江西赣州、广西巴马、湖南芷江等8个种源综合排名靠前,均在聚类分析的第3类中。主成分分析和聚类分析表明浙江江山、广西巴马、广东惠州及江西的赣州、德兴和婺源6个种源,浙江江山、湖南溆浦、江西的赣州和横峰4个种源,湖南溆浦、广东惠州、广西巴马和江西德兴4个种源,分别在浙江富阳、浙江江山和湖南溆浦表现较好,且各主要药效成分含量基本高于均值。【结论】浙江江山、湖南溆浦、广东惠州、江西赣州、广西巴马、湖南芷江等8个种源在异质园基地综合表现均较好,不仅�[Objective]The tea medicine of Lithocarpus litseifolius has a long history.The young leaves are rich in flavonoids,phloridzin and other ingredients.It has attracted much attention because of its significant curative effect on diabetes and other diseases.Based on the fact that introduction is an important way to expand resources,this study intends to use multiple statistical methods to analyze the stability and variation characteristics of the main medicinal components after the introduction of different provenances to multiple heterogeneous garden bases,thus laying a foundation for its introduction and resource utilization.[Method]In this paper,twelve provenances from six provinces were introduced to three heterogeneous garden experimental bases.The total flavonoids,trilobatin,phlorizin,dihydrochalcone and the ratio of trilobatin/phlorizin were determined.Four statistical methods including one-way analysis of variance(one-way ANOVE),multiple comparisons(Duncan's),principal component analysis and cluster analysis are used to compare and analyze the stability,differences,comprehensive scores and cluster characteristics of major medicinal components before and after introduction of Lithocarpus litseifolius.[Result]The study found that the main components similarity of Lithocarpus litseifolius were 0.875-1.000,which confirmed that the main components were relatively stable after introduction.A total of 36 introductions were conducted in this experiment,and the number of introductions of total flavonoids,trilophyls,phlorizin,dihydrochalcone and trilophyllin/phlorizin ratios higher than those in the place of origin were 21,10,27,14 and 5,accounting for 58.33%,27.78%,75.00%,38.89% and 13.89% of the total number of introductions,respectively.Cluster analysis showed that 36test materials were clustered into 4 categories when the Euclidean distance was 5,of which 11 were in the third category.The contents of total flavonoids,trilobatin,phloridzin and dihydrochalcone were higher,mostly higher than those in the origin.In the

关 键 词：木姜叶柯 不同种源 异质园基地 引种 药效成分 

分 类 号：S722.7[农业科学—林木遗传育种]