生物炭添加对红树林湿地土壤细菌群落的影响  

Effects of biochar addition on soil bacterial communities in mangrove wetlands

作　　者：尉鹏雁 游惠明[2,3,4] 谭芳林 郑立地[2,3,4] 乐通潮 刘金福 YU Pengyan;YOU Huiming;TAN Fanglin;ZHENG Lidi;LE Tongchao;LIU Jinfu(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fujian Academy of Forestry Science,Fuzhou 350012,China;National Positional Observatory of Wetland Ecosystems in Quanzhou Bay,Quanzhou,Fujian 362000,China;Key Laboratory of Forestry Cultivation and Forest Product Processing and Utilization of Fujian Province,Fuzhou 350012,China)

机构地区：[1]福建农林大学林学院,福建福州350002 [2]福建省林业科学研究院生态环境研究所,福建福州350012 [3]福建泉州湾湿地生态系统国家定位观测研究站,福建泉州362000 [4]福建省森林培育与林产品加工利用重点实验室,福建福州350012

出　　处：《江西农业大学学报》2025年第1期141-151,共11页Acta Agriculturae Universitatis Jiangxiensis

基　　金：福建省林业攻关项目(2023FKJ02);国家林业和草原应急揭榜挂帅项目(202303);福建省林业科研项目(2022FKJ04)。

摘　　要：【目的】研究滨海湿地红树林生态恢复过程中的生物炭添加下湿地土壤细菌群落结构的变化,对红树林湿地有机碳循环机制研究和红树林湿地碳汇功能提升具有重要意义。【方法】以闽江河口红树林湿地土壤为研究对象,设置3种生物炭(互花米草生物炭、芦苇生物炭和秋茄生物炭)和3种添加量(炭土比分别为1%、2%和4%)处理,通过高通量测序技术研究生物炭添加量对红树林湿地土壤细菌多样性及群落结构的影响。【结果】(1)互花米草生物炭产率和含碳量最高;(2)生物炭添加显著提高了红树林湿地土壤细菌的数量与多样性,以芦苇生物炭1%添加时Shannon指数最大,土壤OTUs数量最多;(3)在门水平上,生物炭添加后红树林湿地土壤细菌的优势门为变形菌门、放线菌门、绿弯菌门、酸杆菌门和拟杆菌门,互花米草生物炭和芦苇生物炭添加增加了放线菌门相对丰度,生物炭添加可以有效降低拟杆菌门相对丰度;在纲水平上,α-变形菌纲、放线菌纲和γ-变形菌纲为主要类群,芦苇生物炭以2%添加时α-变形菌纲相对丰度最高,秋茄生物炭以1%添加时放线菌纲相对丰度最高,生物炭添加有效降低了γ-变形菌纲相对丰度;(4)生物炭添加处理与对照组之间存在67个显著差异种群,未添加生物炭土壤中的拟杆菌属、绿弯菌门、黃桿菌科等的相对丰度显著高于生物炭添加土壤。【结论】红树林湿地土壤细菌群落结构通过不同种类生物炭添加而改变,且土壤细菌群落受生物炭种类以及添加量的影响,生物炭添加可以提高土壤细菌群落的多样性及物种丰富度。[Objective]This study aims to investigate the changes in the bacterial community structure of coastal wetland soils in response to biochar addition during the ecological restoration of mangrove wetlands,which is of significant importance for understanding the organic carbon cycling mechanisms in mangrove wetlands and enhancing their carbon sequestration function.[Method]Using soils from mangrove wetlands in the Min River estuary as the research subject,three types of biochar(Spartina alterniflora biochar,reed biochar,and Kandelia obovata biochar)and three addition levels(biochar-to-soil ratios of 1%,2%,and 4%)were set.The effects of biochar addition on bacterial diversity and community structure were examined through high-throughput sequencing technology.[Result](1)Spartina alterniflora biochar had the highest yield and carbon content;(2)Biochar addition significantly increased the quantity and diversity of bacteria in mangrove wetland soils,with the Shannon index peaking and OTUs being most numerous at 1% reed biochar addition;(3)At the phylum level,the dominant bacterial phyla in mangrove wetland soils after biochar addition were Proteobacteria,Actinobacteria,Chloroflexi,Acidobacteria,and Bacteroidetes.Spartina alterniflora and reed biochar increased the relative abundance of Actinobacteria,while biochar addition effectively reduced the relative abundance of Bacteroidetes.At the class level,Alphaproteobacteria,Actinobacteria,and Gammaproteobacteria were the main groups.The relative abundance of Alphaproteobacteria was the highest with 2% reed biochar addition,while Actinobacteria was the most abundant with 1% Kandelia obovata biochar addition.Biochar addition effectively reduced the relative abundance of Gammaproteobacteria;(4)A total of 67significantly different taxa were identified between the biochar-treated and control groups.The relative abundances of Bacteroides,Chloroflexi,and Flavobacteriaceae were significantly higher in soils without biochar compared with those with biochar amendments.[Conclusion]The

关 键 词：红树林 生物炭 土壤细菌 多样性 群落结构 

分 类 号：S154.3[农业科学—土壤学]