Immobilization of laccase on magnetic PEGDA-CS inverse opal hydrogel for enhancement of bisphenol A degradation in aqueous solution  

作　　者：Mei Du Jingzhang Liu Qiong Wang Fengbang Wang Lei Bi Chunyan Ma Maoyong Song Guibin Jiang 

机构地区：[1]Key Laboratory of Environmental Nanotechnology and Health Effects,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China [2]State Key Laboratory of Environmental Chemistry and Ecotoxicology,Research Center for Eco-Environmental Sciences,Chinese Academy of Science,Beijing 100085,China [3]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Journal of Environmental Sciences》2025年第1期74-82,共9页环境科学学报(英文版)

基　　金：supported by the National Key Research and Development Program of China(Nos.2022YFC3703700 and 2021YFA0910300);the National Natural Science Foundation of China(No.22125606);the Special Project of Ecological Environmental Technology for Carbon Dioxide Emissions Peak and Carbon Neutrality(No.RCEES-TDZ-2021-21).

摘　　要：Endocrine disruptors such as bisphenol A(BPA)adversely affect the environment and human health.Laccases are used for the efficient biodegradation of various persistent organic pollutants in an environmentally safe manner.However,the direct application of free laccases is generally hindered by short enzyme lifetimes,non-reusability,and the high cost of a single use.In this study,laccases were immobilized on a novel magnetic threedimensional poly(ethylene glycol)diacrylate(PEGDA)-chitosan(CS)inverse opal hydrogel(LAC@MPEGDA@CS@IOH).The immobilized laccase showed significant improvement in the BPA degradation performance and superior storage stability compared with the free laccase.91.1%of 100 mg/L BPA was removed by the LAC@MPEGDA@CS@IOH in 3 hr,whereas only 50.6%of BPA was removed by the same amount of the free laccase.Compared with the laccase,the outstanding BPA degradation efficiency of the LAC@MPEGDA@CS@IOH was maintained over a wider range of pH values and temperatures.Moreover,its relative activity of was maintained at 70.4%after 10 cycles,and the system performed well in actual water matrices.This efficientmethod for preparing immobilized laccases is simple and green,and it can be used to further develop ecofriendly biocatalysts to remove organic pollutants from wastewater.

关 键 词：Bisphenol A removal Laccase immobilization CHITOSAN Inverse opal hydrogel 

分 类 号：X703[环境科学与工程—环境工程]