Effects of salinity and betaine addition on anaerobic granular sludge properties and microbial community succession patterns in organic saline wastewater  

作　　者：Zhifei Zhang Jinquan Wan Gang Ye Yan Wang Yuwei Bai Zhicheng Yan 

机构地区：[1]College of Environment and Energy,South China University of Technology,Guangzhou 510006,China [2]Shijiazhuang High Tech Industrial Development Zone Water Supply and Drainage Company,Shijiazhuang 050000,China

出　　处：《Journal of Environmental Sciences》2025年第1期310-321,共12页环境科学学报(英文版)

基　　金：supported by the Guangdong Special Support ProgramProject(No.2021JC060580);the Foshan Innovation Team Project(No.2130218003140).

摘　　要：In this study,the effects of different salinity gradients and addition of compatible solutes on anaerobic treated effluent water qualities,sludge characteristics and microbial communities were investigated.The increase in salinity resulted in a decrease in particle size of the granular sludge,which was concentrated in the range of 0.5-1.0 mm.The content of EPS(extracellular polymeric substances)in the granular sludge gradually increased with increasing salinity and the addition of betaine(a typical compatible solute).Meanwhile,the microbial community structure was significantly affected by salinity,with high salinity reducing the diversity of bacteria.At higher salinity,Patescibacteria and Proteobacteria gradually became the dominant phylum,with relative abundance increasing to 13.53%and 12.16%at 20 g/L salinity.Desulfobacterota and its subordinate Desulfovibrio,which secrete EPS in large quantities,dominated significantly after betaine addition.Their relative abundance reached 13.65%and 7.86%at phylum level and genus level.The effect of these changes on the treated effluent was shown as the average chemical oxygen demand(COD)removal rate decreased from 82.10%to 79.71%,78.01%,68.51%and 64.55%when the salinity gradually increased from 2 g/L to 6,10,16 and 20 g/L.At the salinity of 20 g/L,average COD removal increased to 71.65%by the addition of 2 mmol/L betaine.The gradient elevated salinity and the exogenous addition of betaine played an important role in achieving stability of the anaerobic system in a highly saline environment,which provided a feasible strategy for anaerobic treatment of organic saline wastewater.

关 键 词：Organic saline wastewater Anaerobic granular sludge Compatible solute Extracellular polymeric substance Microbial community 

分 类 号：X70[环境科学与工程—环境工程]