Metagenomic perspectives on antibiotic resistance genes in tap water:The environmental characteristic,potential mobility and health threat  

作　　者：Qiyue Meng Yibo Zhang Da He Yu Xia Jie Fu Chenyuan Dang 

机构地区：[1]School of Environmental Science and Engineering,Huazhong University of Science and Technology,Wuhan 430074,China [2]Key Laboratory of Ecological Impacts of Hydraulic Projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources,Institute of Hydroecology,Ministry of Water Resources&Chinese Academy of Sciences,Wuhan 430074,China [3]School of Environmental Science and Engineering,College of Engineering,Southern University of Science and Technology,Shenzhen 518055,China

出　　处：《Journal of Environmental Sciences》2025年第1期582-596,共15页环境科学学报(英文版)

基　　金：supported by the National Key R&D Program of China(No.2022YFE0103200);the Hubei Provincial Natural Science Foundation of China(No.2021CFB016);the National Natural Science Foundation of China(No.52100217).

摘　　要：As an emerging environmental contaminant,antibiotic resistance genes(ARGs)in tap water have attracted great attention.Although studies have provided ARG profiles in tap water,research on their abundance levels,composition characteristics,and potential threat is still insufficient.Here,9 household tap water samples were collected from the Guangdong-Hong Kong-Macao Greater Bay Area(GBA)in China.Additionally,75 sets of environmental sample data(9 types)were downloaded from the public database.Metagenomics was then performed to explore the differences in the abundance and composition of ARGs.221 ARG subtypes consisting of 17 types were detected in tap water.Although the ARG abundance in tap water was not significantly different from that found in drinking water plants and reservoirs,their composition varied.In tap water samples,the three most abundant classes of resistance genes were multidrug,fosfomycin and MLS(macrolide-lincosamidestreptogramin)ARGs,and their corresponding subtypes ompR,fosX and macB were also the most abundant ARG subtypes.Regarding the potential mobility,vanS had the highest abundance on plasmids and viruses,but the absence of key genes rendered resistance to vancomycin ineffective.Generally,the majority of ARGs present in tap water were those that have not been assessed and are currently not listed as high-threat level ARG families based on the World Health Organization Guideline.Although the current potential threat to human health posed by ARGs in tap water is limited,with persistent transfer and accumulation,especially in pathogens,the potential danger to human health posed by ARGs should not be ignored.

关 键 词：Antibiotic resistance genes Tap water Plasmids Viruses Health threat 

分 类 号：R73[医药卫生—肿瘤]