基于液质及代谢组学方法分析补阳还五汤灌胃大鼠前后血脑中成分差异  

Component differences in blood and brain of rats before and after intragastric administration of Buyang Huanwu Decoction based on liquid chromatography-mass spectrometry and metabolomics approaches

作　　者：温梦昕 李思媛 刘颖芳 陈光宇[1,2] 谢梦洲[1,2] 李亮[1,2] WEN Mengxin;LI Siyuan;LIU Yingfang;CHEN Guangyu;XIE Mengzhou;LI Liang(Hunan Key Laboratory of Chinese Medicine Diagnostics,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;Key Laboratory of Chinese Medicine Heart and Lung Syndrome Differentiation&Medicated Diet and Dietotherapy,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China)

机构地区：[1]湖南中医药大学中医诊断学湖南省重点实验室,湖南长沙410208 [2]湖南中医药大学中医心肺病症辨证与药膳食疗重点研究室,湖南长沙410208

出　　处：《湖南中医药大学学报》2025年第2期250-258,共9页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金面上项目(82374323);湖南省自然科学基金面上项目(2022JJ30435);湖南省卫生健康委科研计划项目(C202302087602);湖南中医药大学研究生创新课题(2023CX180,2024CX153);湖南中医药大学本科生研创新基金(2023BKS024)。

摘　　要：目的研究补阳还五汤(BYHWD)及灌胃给药前后大鼠血清和脑组织中的天然产物成分和内源性代谢产物差异,为该复方的作用机制提供参考。方法将20只大鼠平均分为2组,中药组(25.65 g·kg^(-1))和空白组(给予等量生理盐水)各10只,每天给药1次,连续给药3 d,第4天给药2 h后,腹主动脉取血并断头取脑组织。采用超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q-TOF-MS)技术,对BYHWD、正常大鼠血清、脑组织和灌胃给药大鼠血清、脑组织天然产物及代谢产物进行分析。结果从BYHWD中共指认出90个天然产物成分;大鼠灌胃BYHWD后,发现25个入血成分,10个入脑成分,其中5个成分能同时入血入脑。代谢组学分析发现,血清内源性差异成分主要涉及亚油酸代谢、类固醇激素生物合成、苯丙氨酸代谢等8条代谢通路。脑组织内源性代谢差异成分主要涉及视黄醇代谢、类固醇激素生物合成、咖啡因代谢等9条代谢途径。结论通过分析BYHWD的天然产物成分、灌胃大鼠前后血脑中成分差异及相关代谢途径,可为阐明BYHWD有效成分及其作用机制提供参考。Objective To investigate the differences in natural product components and endogenous metabolites in rat serum and brain tissue before and after intragastric administration of Buyang Huanwu Decoction(BYHWD),and to provide a reference for elucidating the mechanism of action of this Chinese compound formula.Methods Twenty rats were equally divided into two Chinese medicines group(administered with BYHWD at 25.65 g·kg^(-1))and blank group(administered with an equal volume of saline),with ten rats in each group.The medicine was administered once daily for three consecutive days.On the fourth day,two hours after administration,blood was collected from the abdominal aorta and brain tissue was obtained by decapitation.Ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS)was used to analyze the natural products and metabolites in BYHWD,as well as in the serum and brain tissue of normal rats and rats after intragastric administration of BYHWD.Results Ninety natural product components were identified in BYHWD.After intragastric administration of BYHWD to rats,25 components were found in the serum and ten in the brain tissue,with five components detected in both.Metabolomics analysis revealed that the endogenous differential components in serum primarily involved in eight metabolic pathways,including linoleic acid metabolism,steroid hormone biosynthesis,and phenylalanine metabolism.The endogenous metabolic differential components in the brain tissue were primarily associated with nine metabolic pathways,including retinol metabolism,steroid hormone biosynthesis,and caffeine metabolism.Conclusion By analyzing the natural product components of BYHWD,the differences in components in the serum and brain tissue of rats before and after intragastric administration,and the related metabolic pathways,this study provides a reference for elucidating the active components of BYHWD and its mechanism of action.

关 键 词：补阳还五汤 超高效液相色谱-四极杆-飞行时间质谱 代谢组学 天然产物 内源性代谢产物 

分 类 号：R284[医药卫生—中药学]