试剂爆珠应用于野战输血相容性检测的效果评价  

Evaluation of effectiveness of reagent burstbead applied to field blood transfusion compatibility testing

作　　者：张怡 刘晓娣 赵义曼 周景艳 杨书梅 闫浩 马印图 ZHANG Yi;LIU Xiao-di;ZHAO Yi-man;ZHOU Jing-yan;YANG Shu-mei;YAN Hao;MA Yin-tu(The 980th Hospital of Joint Logistics Support Force,Shijiazhuang 050082,China)

机构地区：[1]联勤保障部队第980医院,石家庄050082

出　　处：《医疗卫生装备》2025年第2期39-42,共4页Chinese Medical Equipment Journal

基　　金：河北省医学科学研究计划课题(20241160)。

摘　　要：目的:采用试剂爆珠进行血型鉴定、抗体筛查以及交叉配血试验,并与现有检测方法进行平行对比测试,评价试剂爆珠应用于野战输血前相容性检测的可靠性。方法:选择野外救治方舱作为试验点,模拟野战血站条件下伤员急救输血的场景,对某院1358例输血前送检的血液样本分别采用爆珠法(应用试剂爆珠)和试管法进行血型鉴定平行对比试验,采用爆珠法与手工聚凝胺法进行抗体筛查和交叉配血平行对比试验,并比较采用爆珠法和其他方法下血型鉴定检测结果的符合率、抗体筛查阳性样本检出率和交叉配血不合的情况。结果:爆珠法和试管法血型鉴定试验的符合率为100%,二者一致性良好。在抗体筛查和交叉配血试验中,爆珠法检出5例抗筛阳性导致主侧配血不合的样本(阳性率为0.37%),而手工聚凝胺法仅检测出2例不规则抗体阳性样本(阳性率为0.15%),2种方法均检测出3例次侧配血不合。结论:采用试剂爆珠进行血型鉴定、抗体筛查以及交叉配血试验,准确性和可靠性高,适用于野战条件下进行输血相容性检测。Objective To explore the reliability of the reagent burstbead for field blood transfusion compatibility testing by applying it to bloodtype detection,antibody screening and cross matching tests and conduting parallel contrast with the existing arrays.Methods A field treatment shelter was used as the testing site to simulate the scene of emergency blood transfusion for the wounded under the conditions of field blood station,and 1358 pieces of blood samples sent to some hospital for examination before transfusion were considered as the objects.Parallel contrast tests were carried out between the reagent burstbead method and the tube method for bloodtype detection and between the reagent burstbead method and the manual polybrene method for antibody screening and cross matching.The reagent burstbead method was compared with other methods in terms of the coin-cidence rate of the bloodtype detection results,the detection rate of positive samples for antibody screening and crossmatch incompatibility.Results The reagent burstbead method and the tube method had high consistency when used for bloodtype detection,with the coincidence rate being 100%.During antibody screening and cross matching tests,the reagent burstbead method found 5 cases of antibody-positive samples leading to major crossmatch incompatibility(positive rate being 0.37%),the manual polybrene method detected 2 cases of irregular antibody-positive samples(positive rate being 0.15%),and the two methods both identified 3 cases of minor crossmatch incompatibility.Conclusion The reagent burstbead has high accuracy and reliability when applied to bloodtype detection,antibody screening and cross matching tests,and thus can be used for blood transfusion compatibility testing in field conditions.

关 键 词：试剂爆珠 野战输血 输血相容性检测 血型鉴定 抗体筛查 交叉配血 

分 类 号：R318[医药卫生—生物医学工程] R82[医药卫生—基础医学]