Hunting for a viral proxy in bioaerosols of swine buildings using molecular detection and metagenomics  

作　　者：Valérie Létourneau Marie-Josée Gagné Jonathan M.Vyskocil Vincent Brochu Kim Robitaille Martin Gauthier Julie Brassard Caroline Duchaine 

机构地区：[1]Quebec Heart and Lung Institute Research Centre-UniversitéLaval,2725 Chemin Sainte-Foy,Quebec,G1V 4G5,Canada [2]Saint-Hyacinthe Research and Development Centre,Agriculture and Agri-Food Canada,3600 Casavant Boulevard West,Saint-Hyacinthe,J2S 8E3,Canada [3]Department of Biochemistry,Microbiology,and Bio-informatics,Faculty of Science and Engineering,UniversitéLaval,1045 Avenue de la Médecine,Quebec,G1V 0A6,Canada [4]The Swine and Poultry Infectious Diseases Research Centre,Faculty of Veterinary Medicine of Universitéde Montréal,3200 Rue Sicotte,Saint-Hyacinthe,J2S 2M2,Canada

出　　处：《Journal of Environmental Sciences》2025年第2期69-78,共10页环境科学学报(英文版)

摘　　要：There are limited biosecurity measures directed at preventing airborne transmission of viruses in swine.The effectiveness of dust mitigation strategies such as oil sprinkling,to decrease risk of airborne virus transmission are unknown.Metagenomics and qPCR for common fecal viruses were used to hunt for a ubiquitous virus to serve as a proxy when evaluating the efficiency of mitigation strategies against airborne viral infectious agents.Air particles were collected from swine buildings using high-volume air samplers.Extracted DNA and RNA were used to perform specific RT-qPCR and qPCR and analyzed by highthroughput sequencing.Porcine astroviruses group 2 were common(from 102 to 105 genomic copies per cubic meter of air or gc/m^(3),93%positivity)while no norovirus genogroup II was recovered from air samples.Porcine torque teno sus virus were detected by qPCR in low concentrations(from 101 to 102 gc/m^(3),47%positivity).Among the identified viral families by metagenomics analysis,Herelleviridae,Microviridae,Myoviridae,Podoviridae,and Siphoviridae were dominant.The phage vB_AviM_AVP of Aerococcus was present in all air samples and a newly designed qPCR revealed between 101 and 105 gc/m^(3) among the samples taken for the present study(97%positivity)and banked samples from5-and 15-year old studies(89%positivity).According to the present study,both the porcine astrovirus group 2 and the phage vB_AviM_AVP of Aerococcus could be proxy for airborne viruses of swine buildings.

关 键 词：BIOAEROSOLS Viral metagenomics ASTROVIRUS Aerococcus phage Swine buildings 

分 类 号：S85[农业科学—兽医学]