新资源食品云威灵化学成分分析及促消化功能比较  

UPLC-Q-TOF-MS/MS-based Phytochemical Characterization and Comparative of Digestive Promotion Prospective of a New Resource Food Inula nervosa

作　　者：陈恭 金琼 龚冰璐 高世佳 高瑞 谭文红 刘录 CHEN Gong;JIN Qiong;GONG Binglu;GAO Shijia;GAO Rui;TAN Wenhong;LIU Lu(School of Ethnic Medicine,Yunnan University of Chinese Medicine,Kunming 650500,China;Yunnan University and Technology Research Center of Health Food and Cosmetics,Kunming 650500,China)

机构地区：[1]云南中医药大学民族医药学院,云南昆明650500 [2]云南省高校保健食品化妆品工程研究中心,云南昆明650500

出　　处：《现代食品科技》2025年第1期28-36,共9页Modern Food Science and Technology

基　　金：云南省生物医药重大专项(202202AA100009;202302AA310006);云南省高校工程研究中心(2020YGG01);云南省高校重点实验室(2022YGZ02)。

摘　　要：该文建立了稳定可靠的快速分析云威灵化学成分的方法,并对比了云威灵水提物和挥发油的促消化功能。采用超高压液相-三重四级杆-飞行时间质谱联用仪分析新资源食品云威灵(Inula nervosa Wall.)化学成分,通过保健食品功能评价方法测定小鼠小肠运动、胃泌素(GAS)、胃动素(MTL)、大鼠体质量增长、摄食量、食物利用率、胃蛋白酶活性及排出量来对比云威灵水提物和挥发油的促消化效果。结果表明,质谱分析共鉴定出18个化合物。水提物高剂量组及挥发油低、中、高剂量组小鼠小肠推进率提高了58.61%、49.27%、61.88%、83.78%(P<0.05,P<0.05,P<0.01,P<0.0001),且能显著提高GAS、MTL的分泌(P<0.05)。水提物低、中、高剂量组大鼠摄食量提高了6.05%、6.99%、8.91%(P<0.05,P<0.01,P<0.0001),挥发油中、高剂量组提高6.72%、8.74%(P<0.01,P<0.001)。水提物和挥发油均能使胃蛋白酶活性显著增强(P<0.01),但挥发油高剂量组与水提物高剂量组相比具有统计学意义(P<0.01)。两者均能增加胃蛋白酶排出量(P<0.05)。云威灵水提物及挥发油均具有促消化效果且挥发油更优。A stable and reliable method for the rapid analysis of the chemical components of the new resource food of Inula nervosa Wall.was established.The digestion-promoting functions were compared across diverse solvents(water and oil)for I.nervosa.The chemical components of the new resource food I.nervosa were analyzed using UPLC-Q-TOF-MS/MS.According to the principles of functional food,the distance of ink movement in the small intestine,GAS,and MTL in mice,body weight,food intake,food efficiency ratio,secretion amount of pepsin,and pepsin activity were evaluated.The results revealed 18 relevant compounds.The small intestine motility of mice in the high-dose water extract group,low,medium,and high-dose volatile oil groups increased by 58.61%,49.27%,61.88%,and 83.78%(P<0.05,P<0.05,P<0.01,P<0.0001),respectively.These results notably led to an increase in the secretion of GAS and MTL(P<0.05).Food intake in the low,medium,and high-dose water extract groups increased by 6.05%,6.99%,and 8.91%(P<0.05,P<0.01,P<0.0001)and in medium and high-dose volatile oil groups by 6.72% and 8.74%(P<0.01,P<0.001),respectively.All extraction methods greatly enhanced pepsin activity(P<0.01).However,there was a notable difference between high-dose volatile oil and highdose water extract(P<0.01).Pepsin secretions significantly increased in both groups(P<0.05);water extract and volatile oil exhibited digestion-enhancing effects,with volatile oil exhibiting superior effects compared to the water extract.

关 键 词：云威灵 化学成分 超高压液相-三重四级杆-飞行时间质谱联用仪 促消化作用 胃蛋白酶 

分 类 号：R28[医药卫生—中药学]