蝉棒束孢C1菌丝体多糖抑制MAPK/NF-κB信号通路缓解LPS诱导的RAW264.7细胞炎症反应  

Anti-inflammatory Effects of Polysaccharides from Isaria cidada Miq.C1 Mycelial through the MAPK/NF-κB Signaling Pathways in RAW264.7 cell induced by LPS

作　　者：邵丽 牛珂欣 刘惠 蒋虹 贾庆文 马来记 SHAO Li;NIU Kexin;LIU Hui;JIANG Hong;JIA Qingwen;MA Laiji(College of Perfume and Aroma Technology,Shanghai Institute of Technology,Shanghai 201418,China;Shandong Freda Biotech Co.Ltd.,Jinan 250101,China)

机构地区：[1]上海应用技术大学香料香精化妆品学部,上海201418 [2]山东福瑞达生物股份有限公司,山东济南250101

出　　处：《现代食品科技》2025年第1期47-56,共10页Modern Food Science and Technology

基　　金：香料香精化妆品省部共建协同创新中心开放课题(1021ZK230016013)。

摘　　要：该文研究蝉棒束孢(Isaria cidada Miquel)C1菌丝体多糖ISP-80对脂多糖(LPS)诱导的小鼠单核巨噬细胞RAW264.7炎症模型的抗炎效果,并探讨其作用机制。以热水浸提法获得菌丝体多糖ISP-80,采用CCK-8法测定ISP-80对RAW264.7细胞的毒性作用,使用ELISA试剂盒测定TNF-α、IL-1β、IL-6细胞因子分泌量,通过Western Blot法检测MAPK/NF-κB信号通路中相关蛋白的含量。结果显示,ISP-80多糖提取率为10.42%,共含有3种多糖组分,由葡萄糖(Glc)、甘露糖(Man)、半乳糖(Gal)3种单糖组成,摩尔比为1.00:5.03:1.55。ISP-80多糖在添加0.01~1.00 mg/mL质量浓度范围内时对细胞无毒性且能促进细胞增殖(P<0.0001)。0.25 mg/mL ISP-80处理组可显著降低促炎细胞因子IL-1β(P<0.001)和IL-6(P<0.05)的分泌,分别降至LPS组的0.59倍和0.88倍,且可明显抑制细胞分化,恢复细胞形态。ISP-80的抗炎作用是通过MAPK和NF-κB途径介导的,ISP-80通过阻断p-p38和p-JNK来抑制MAPK途径,分别降低了0.20和0.41(P<0.001),并且通过阻断IκBα和p65来抑制NF-κB通路,分别降低了0.43(P<0.01)和0.24(P<0.001)。因此,ISP-80具有较好的抗炎作用,可作为预防和治疗炎症性疾病的天然和安全的抗炎剂。The anti-inflammatory effects and mechanisms of the mycelial polysaccharide ISP-80 from the Isaria cicada Miquel C1 was investigated in a mouse monocyte-macrophage RAW264.7 inflammation model,induced by lipopolysaccharide(LPS).The polysaccharide(ISP-80)was obtained through hot water extraction from the Isaria cidada C1 mycelium.The toxic effects of ISP-80 on RAW264.7 cells were evaluated using the CCK-8 assay,and the impact of ISP-80 on the secretion of TNF-α,IL-1β,and IL-6 cytokines was measured using ELISA.Furthermore,the mechanism of ISP-80 was analyzed using western blotting to detect the relevant proteins in the MAPK/NF-κB pathways.The findings indicated that the extraction rate of ISP-80 was 10.42%and it was composed of three polysaccharide fractions,each containing Glc,Man,and Gal monosaccharides,in a molar ratio of 1.00:5.03:1.55.Adding ISP-80 at 0.01~1.00 mg/mL exerted no cytotoxic effects and promoted cell proliferation(P<0.0001).The 0.25 mg/mL ISP-80 treatment significantly decreased the secretion of pro-inflammatory cytokines IL-1β(P<0.001)and IL-6(P<0.05),reaching 0.59-and 0.88-times those of the LPS group,respectively,and inhibited cell differentiation while restoring cell morphology.The anti-inflammatory action of ISP-80 acted via the MAPK/NF-κB pathways.ISP-80 inhibited the MAPK pathway by blocking p-p38 and p-JNK,which decreased by 0.20 and 0.41,respectively(P<0.001).Furthermore,the NF-κB pathway was inhibited by blocking IκBαand p65,which decreased by 0.43(P<0.01)and 0.24(P<0.001),respectively.These results indicate that ISP-80 can be a natural and safe antiinflammatory agent for the prevention and treatment of inflammatory diseases.

关 键 词：蝉棒束孢C1 多糖 抗炎作用 MAPK通路 RAW264.7巨噬细胞 

分 类 号：R28[医药卫生—中药学]