铁皮石斛毛兰素对人食管癌KYSE-150细胞的抑制作用  

Inhibitory Effect of Erianin from Dendrobium officinale on Human Esophageal Cancer KYSE-150 Cells

作　　者：燕文柏 赵庆俊 胡艺萍 罗纪红 邓晓东 赵志伟 魏玉颖 蒋璐遥 严阳 周俊含 甘菊丽 杜彦锋 张薇薇 YAN Wenbai;ZHAO Qingjun;HU Yiping;LUO Jihong;DENG Xiaodong;ZHAO Zhiwei;WEI Yuying;JIANG Luyao;YAN Yang;ZHOU Junhan;GAN Juli;DU Yanfeng;ZHANG Weiwei(School of Public Health,Chengdu Medical College,Chengdu 610500,China;The First Affiliated Hospital of Traditional Chinese Medicine of Chengdu Medical College,Xindu Hospital of Traditional Chinese Medicine,Chengdu 610500,China)

机构地区：[1]成都医学院公共卫生学院,四川成都610500 [2]成都医学院第一附属中医医院,成都市新都区中医医院,四川成都610500

出　　处：《现代食品科技》2025年第1期57-64,共8页Modern Food Science and Technology

基　　金：四川中医药大健康产业发展与乡村振兴研究中心一般项目(DJKYB202302);成都医学院医院发展研究中心项目(YYFZ23001);成都医学院省级大学生创新创业训练计划项目(S202213705064);成都医学院国家级、省级大学生创新创业训练计划(202313705027);成都医学院研究生科研创新基金一般项目(YCX2023-01-49);重大疾病靶点发现与蛋白药物研发四川省高校重点实验室项目(23LHZY04);四川省中医药管理局中医药科研专项课题一般项目(2024MS232)。

摘　　要：该研究旨在探明铁皮石斛毛兰素对人食管癌KYSE-150细胞生长的抑制作用。分别采用CCK-8试剂盒、显微镜观察、划痕实验和流式细胞技术检测毛兰素对KYSE-150细胞的活性,细胞形态变化、细胞迁移能力和细胞凋亡、周期阻滞的影响,并通过Western Blot法检测Bax、Bcl-2、Vimentin、Akt、P-Akt、PI3K、P-PI3K、N-cadherin、E-cadherin蛋白的表达情况。结果表明,毛兰素对KYSE-150细胞生长具有抑制作用,24、48、72 h的IC50分别为357.30、120.20、57.43 nmol/L,细胞出现皱缩、聚团生长现象,细胞迁移能力降低,同时与对照组相比,40、80、120 nmol/L作用24 h后,细胞凋亡百分比分别是27.57%、35.38%、61.97%,处理组细胞G1期比例明显降低,由56.64%下降至2.80%,G2期细胞比例明显增加,由16.82%增加至86.98%,Western Blot实验中加药组Bax和E-cadherin表达相对增高,Bcl-2、Vimentin、P-Akt、PI3K、P-PI3K、N-cadherin蛋白表达相对降低,表明毛兰素可诱导细胞凋亡,抑制细胞迁移。由此可见,铁皮石斛毛兰素可通过抑制EMT途路抑制细胞迁移,通过调控PI3K/Akt信号通路抑制KYSE-150细胞增殖并诱导其凋亡。The inhibitory effect of erianin extracted from Dendrobium officinale on the growth of human esophageal cancer KYSE-150 cells was investigated.The CCK-8 kit,microscope,scratch test,and flow cytometry were used to determine the inhibitory effect,morphological changes,cell migration,cell apoptosis,and cycle arrest of erianin on the activity of KYSE-150 cells.Western blot analysis was used to detect the expression levels of Bax,Bcl-2,Vimentin,Akt,P-Akt,PI3K,P-PI3K,N-cadherin,and E-cadherin proteins.The results of cell experiments showed that the IC50 of erianin for inhibiting the growth of KYSE-150 cells at 24,48,and 72 h was 357.30,120.20,and 57.43 nmol/L,respectively.The cells exhibited reduced migration capability and demonstrated shrinkage and aggregation during growth.After treatment with 40,80,and 120 nmol/L for 24 h,the percentage of apoptosis was found to be 27.57%,35.38%,and 61.97%,respectively,compared with the control group.The proportion of cells in the G1 phase in the treatment group significantly decreased from 56.64% to 2.80%,whereas the proportion of cells in the G2 phase significantly increased from 16.82% to 86.98%.The western blot results showed that the expression of Bax and E-cadherin increased,whereas the expression of Bcl-2,Vimentin,P-Akt,PI3K,P-PI3K,and N-cadherin decreased,indicating that erianin has the potential to induce apoptosis and inhibit cell migration.In summary,erianin from D.officinale has the capability to inhibit cell migration through the inhibition of the EMT pathway and KYSE-150 cell proliferation and induce apoptosis via regulation of the PI3K/Akt signaling pathway.

关 键 词：铁皮石斛 毛兰素 人食管癌KYSE-150细胞 PI3K/AKT信号通路 

分 类 号：R73[医药卫生—肿瘤]