电子束辐照多花黄精HPLC指纹图谱及抗氧化谱-效关系分析  

HPLC Fingerprint of Electron Beam Irradiation of Polygonatum cyrtonema Hua and Its Antioxidant Spectrum-effect Relationship

作　　者：林永杰 贾鑫 周瑞 王丹[1] 黄敏 高鹏 LIN Yongjie;JIA Xin;ZHOU Rui;WANG Dan;HUANG Min;GAO Peng(School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621010,China;Sichuan Institute of Atomic Energy,Irradiation Preservation Key Laboratory of Sichuan Province,Chengdu 610101,China)

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010 [2]四川原子能研究院,辐照保藏四川省重点实验室,四川成都610101

出　　处：《现代食品科技》2025年第1期221-228,共8页Modern Food Science and Technology

基　　金：四川省科技创新人才项目(2023JDRC0042);四川省区域创新合作项目(2022YFQ0044)。

摘　　要：该研究以多花黄精为试材,建立多花黄精的HPLC指纹图谱,提取特征指纹图谱信息,结合相似度评价、聚类分析、正交偏最小二乘判别分析(OPLS-DA)对多花黄精药材进行综合分析,并筛选差异性标志物质。以DPPH、ABTS+自由基清除率评价多花黄精抗氧化活性,结合偏最小二乘回归分析(PLSR)的方法探究指纹图谱与抗氧化活性之间的谱-效关系。结果表明不同剂量辐照多花黄精HPLC指纹图谱相似度在0.972~0.994之间,确定19个共有峰,与标准品对照指认峰2为芦丁,峰9为槲皮素,峰10为甘草素。OPLS-DA筛选出包括芦丁、甘草素、槲皮素等9个辐照前后标志性差异物质。谱效分析表明13个组分与抗氧化活性贡献呈正相关,其中峰1、峰6和芦丁是多花黄精中的强抗氧化物质。该研究反映了不同剂量电子束辐照多花黄精的成分差异和自由基清除能力情况,可有效用于电子束辐照多花黄精的质量监测。Polygonatum cyrtonema Hua was used as the test material to establish the HPLC fingerprint and extract its characteristic fingerprint information.Similarity evaluation,cluster analysis,and orthogonal partial least squares discriminant analysis(OPLS-DA)methods were employed to comprehensively analyze the irradiation of Polygonatum cyrtonema Hua with different doses of electron beams and screen for differential marker substances.The antioxidant activity of Polygonatum cyrtonema Hua was evaluated through DPPH and ABTS+free radical scavenging rates.The spectrum-effect relationship between the fingerprint and antioxidant activity was explored using partial least squares regression analysis(PLSR).The results indicated that the similarity of HPLC fingerprints of Polygonatum cyrtonema Hua ranged from 0.972 to 0.994 at different doses,with 19 common peaks identified.Peak 2 was identified as rutin,peak 9 as quercetin,and peak 10 as liquiritin.OPLS-DA identified nine landmark differential substances before and after irradiation,including rutin,liquiritin,and quercetin.Spectrum-effect analysis demonstrated that 13 components were positively correlated with antioxidant activity,with Peak 1,Peak 6,and rutin identified as strong antioxidant substances in Polygonatum cyrtonema Hua.This study reflected the differences in composition and free radical scavenging ability with different doses of electron beam irradiation of Polygonatum cyrtonema Hua,providing effective data for quality monitoring of electron beam irradiation of Polygonatum cyrtonema Hua.

关 键 词：多花黄精 电子束辐照 HPLC指纹图谱 化学计量学 谱-效关系 

分 类 号：R284.1[医药卫生—中药学]