高效液相色谱-串联质谱法测定化妆品中四氢咪唑啉等5种组分  

Determination of 5 Components in Cosmetics by HPLC-MS/MS,Including Tetrahydrozoline

作　　者：岑立怡 伍英英 欧阳淑娟 梁静文 Cen Liyi;Wu Yingying;Ouyang Shujuan;Liang Jingwen(Guangdong Institute for Drug Control,Guangzhou 510670,China)

机构地区：[1]广东省药品检验所,广东广州510670

出　　处：《广东化工》2025年第2期142-145,共4页Guangdong Chemical Industry

基　　金：广东省药品监督管理局科技创新引导扶持(2022YDZ05);广东省药品监督管理局科技创新团队专项(2023TDZ05)。

摘　　要：建立高效液相色谱-串联质谱法测定化妆品中四氢咪唑啉等5种组分的分析方法。化妆品经饱和氯化钠或正己烷涡旋分散均匀,乙腈提取,高速离心后,取一半清液加水定容,经0.22μm滤膜过滤,采用Inertsil ODS-SP-C_(18)(3.0 mm×150 mm×3μm)色谱柱分离,流动相为0.1%甲酸水溶液-乙腈,流速为0.3 mL/min进行梯度洗脱。使用电喷雾正离子源(ESI+),外标定量,多反应监测(MRM)扫描检测。结果表明四氢咪唑啉、萘甲唑啉、羟甲唑啉、安他唑啉和赛洛唑啉具有良好的线性关系,相关系数在0.99以上。对选取不同基质的三个水平进行加标回收,数值范围为80.6%~110.3%,相对标准偏差(RSD)均在0.7%~5.6%。该方法操作简单,灵敏度高,适用于化妆品中四氢咪唑啉等5种组分的检测。To establish a HPLC-MS/MS method for the determination of five components in cosmetics,including tetrahydrozoline.The cosmetics were dispersed uniformly by saturated sodium chloride or n-hexane vortex,extracted by acetonitrile,centrifuged at high speed,and then half of the clear liquid was fixed with water and filtered through 0.22μm membrane.The separation was performed on an Inertsil ODS-SP-C_(18) column(3.0 mm×150 mm×3μm),with 0.1%formic acid-acetonitrile as mobile phase,gradient elution,and flow rate of 0.3 mL/min.The electrospray positive ion source(ESI+)was used for detection,and the external standard method was used for quantification.The results revealed that the linearity of tetrahydrozoline,naphazoline,oxymetazoline,antazoline and xylometazoline was good within the test range,and the correlation coefficients were all greater than 0.99.The spiked recoveries of different substrates were investigated at three levels.The spiked recoveries were 80.6%~110.3%,and the relative standard deviations(RSDs)were 0.7%~5.6%.This method has high sensitivity,simple operation,and is generally applicable in the detection of cosmetic ingredients.

关 键 词：高效液相色谱-串联质谱 化妆品 四氢咪唑啉 萘甲唑啉 羟甲唑啉 安他唑啉 赛洛唑啉 

分 类 号：TQ658[化学工程—精细化工]