基于温和预处理玉米秸秆的酶解糖化工艺研究  

Research on enzymatic hydrolysis and saccharification process of corn straw based on mild pretreatment

作　　者：张欢欢 林钰程 王圆圆 赵世强[1] 杜朝军[2] 赵子高 常春[1,4] Zhang Huanhuan;Lin Yucheng;Wang Yuanyuan;Zhao Shiqiang;Du Chaojun;Zhao Zigao;Chang Chun(School of Chemical Engineering,Zhengzhou University,Zhengzhou 450001,China;Nanyang Research Institute of Zhengzhou University,Nanyang Institute of Technology,Nanyang 473004,China;National Key Laboratory of Biobased Transport Fuel Technology,Nanyang 473000,China;Henan Center for Outstanding Overseas Scientists,Zhengzhou 450001,China)

机构地区：[1]郑州大学化工学院,河南郑州450001 [2]南阳理工学院郑州大学南阳研究院,河南南阳473004 [3]生物基运输燃料技术全国重点实验室,河南南阳473000 [4]河南省杰出外籍科学家工作室,河南郑州450001

出　　处：《可再生能源》2025年第2期143-151,共9页Renewable Energy Resources

基　　金：国家自然科学基金项目(22178328);南阳市协同创新重大专项(郑州大学南阳研究院)(21XTCX12002);河南省科技攻关项目(222102320059)。

摘　　要：秸秆类生物质的预处理和酶解是实现其高值化转化、利用的关键技术之一。文章以玉米秸秆为原料,对甲酸、亚氯酸钠和碱性过氧化氢预处理方法进行了研究和比较。采用扫描电镜、X射线衍射和傅里叶变换红外光谱等方法对预处理前后玉米秸秆的组成、形貌、晶体结构和官能团进行了分析。经过甲酸、亚氯酸钠和碱性过氧化氢预处理后,木质素去除率分别为66.71%,97.12%和91.88%,酶解率分别达到63.26%,71.83%和95.14%。在纤维素酶添加量为19.6 FPU/g,木聚糖酶添加量为35.44 IU/g,吐温80添加量为20.96 mg/g的条件下,碱性过氧化氢预处理后玉米秸秆的预测酶解率为95.57%,实际酶解率为94.42%。高固体酶解的最佳补料策略是初始底物浓度为8%,在6,12,24 h分别添加4%的底物,酶解120 h后,酶解率达到87.57%。Pretreatment and enzymatic hydrolysis of straw-type biomass are among the key technologies for its high-value conversion and utilization.Using corn stover as the raw material,pretreatment methods with formic acid,sodium chlorite,and alkaline hydrogen peroxide were studied and compared.Methods such as scanning electron microscopy,X-ray diffraction,and Fourier-transform infrared spectroscopy were employed to analyze the composition,morphology,crystal structure,and functional groups of corn straw before and after pretreatment.After pretreatment with formic acid,sodium chlorite,and alkaline hydrogen peroxide,the lignin removal rates were 66.71%,97.12%,and 91.88%respectively,and the enzymatic hydrolysis rates reached 63.26%,71.83%,and 95.14%respectively.Under the conditions where the cellulase dosage is 19.6 FPU/g,the xylanase dosage is 35.44 IU/g,and the Tween 80 dosage is 20.96 mg/g,the predicted enzymatic hydrolysis rate of corn straw pretreated with alkaline hydrogen peroxide is 95.57%,while the actual enzymatic hydrolysis rate is 94.42%.The optimal feeding strategy for high-solid enzymatic hydrolysis was an initial substrate concentration of 8%,with 4%of the substrate added at 6,12 h,and 24 h respectively.After 120 h of enzymatic hydrolysis,the enzymatic hydrolysis rate reached 87.57%.

关 键 词：玉米秸秆 预处理 高固酶解 分批补料 

分 类 号：TK6[动力工程及工程热物理—生物能]