樟树“赣彤1号”在不同光质下扦插生根的转录组分析  

Transcriptome analysis of cutting rooting of Cinnamomum camphora ‘Gantong 1’ under different light qualities

作　　者：万强 胡盼 刘淑娟[2] 刘腾云[2] 姜雪茹 周华[2] WAN Qiang;HU Pan;LIU Shu-juan;LIU Teng-yun;JIANG Xue-ru;ZHOU Hua(College of Forestry,Jiangxi Agricultural University,Nanchang 330045,China;Institute of Biological Resources,Jiangxi Academy of Sciences/Jiangxi Provincial Key Laboratory of Improved Variety Breeding and Efficient Utilization of Native Tree Species,Nanchang 330096,China)

机构地区：[1]江西农业大学林学院,南昌330045 [2]江西省科学院生物资源研究所/江西乡土树种良种选育与高效利用江西省重点实验室,南昌330096

出　　处：《西南农业学报》2024年第11期2370-2380,共11页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(42367007);中央引导地方科技发展资金项目(20192ZDD01004)。

摘　　要：【目的】研究樟树“赣彤1号”在不同光质下扦插的变化情况及其生根的分子机制,为全面揭示“赣彤1号”插穗生根机理奠定理论基础。【方法】以扦插后在4种光质下处理不同时间(0、30、68 d)的插穗基部韧皮部组织为材料,观察“赣彤1号”生根形态,同时对其在不同光质和不同时期进行转录组测序,并对其结果进行基因功能注释和差异表达基因筛选。【结果】红光处理下的生根率大于其他3种光质,红蓝光与白光发育进程较为相似,蓝光处理下生根率最低;在生根发育过程中共发现39 026个差异表达基因,其中,上调23 414个,下调15 612个,红光下差异基因最多,上调6484个,下调3902个;GO富集结果表明,DEGs在次级代谢过程、苯丙醇代谢过程、光合作用、膜锚固构件、叶绿素结合等过程得到显著富集。KEGG结果表明,差异表达基因主要被富集到光合作用、生长素及糖酵解/糖异生等代谢过程。光系统I的氧化还原状态在蓝光下会被抑制,ATP合酶的活性显著下降,蓝光降低了光合作用的效率;糖酵解通路中磷酸葡萄糖酶、果糖-二磷酸醛缩酶等基因在红蓝光下显著上调;在红光及红蓝光下,其生长素水平显著上调。【结论】通过对“赣彤1号”在不同光质下扦插生根的转录组进行分析,结果显示红光下多种基因上调表达,有利于促进其不定根的生长发育。【Objective】By investigating the changes of Cinnamomum camphora ‘Gantong 1' cuttings under different light qualities, the study aimed to establish a theoretical foundation for understanding the rooting mechanism in ‘Gantong 1' cuttings.【Method】The basal cambial tissues of ‘Gantong 1' cuttings treated under four light qualities at different times(0,30,68 days) were utilized as the experimental materials.Rooting morphology of ‘Gantong 1' was observed and transcriptome sequencing was conducted on cuttings exposed to different light qualities and time intervals.Gene functional annotation and differential gene expression analysis were performed.【Result】The rooting rate was higher under red light compared to the other light qualities, while the developmental process under red-blue light and white light appeared relatively similar.The lowest rooting rate was observed under blue light treatment.A total of 39 026 differentially expressed genes were identified throughout the entire rooting process, of which 23 414 were up-regulated and 15 612 were down-regulated.Notably the highest number of differentially expressed genes were observed under red light condition, of which 6484 were up-regulated and 3902 were down-regulated.The gene ontology(GO) function annotation showed that differentially expressed genes were significantly enriched in metabolic pathways such as secondary metabolic pathways, phenylpropanol metabolism, photosynthesis, membrane anchoring components, chlorophyll binding, and others.The KEGG pathway enrichment results revealed that differentially expressed genes mainly responded to photosynthesis, auxin and glycolysis/glycolysis.The redox state of photosystem I was inhibited under blue light treatment, the activity of ATP synthase was significantly decreased, and blue light was shown to diminish the efficiency of photosynthesis.The genes of the glycolytic pathway, phosphoglucomutase and fructose-bisphosphate aldolase, were significantly up-regulated under red-blue light treatment, and the le

关 键 词：光质 扦插生根 转录组 差异表达基因 

分 类 号：S792.23[农业科学—林木遗传育种]