显齿蛇葡萄与广东蛇葡萄二氢杨梅素积累机制研究  

Accumulation mechanism of dihydromyricetin in Ampelopsis grossedentata and A.cantoniensis

作　　者：朱倩锋 徐楠 杜丽桦 夏敏 陈彦超 汪启明[1] 于华忠[2] 饶力群[1] ZHU Qian-feng;XU Nan;DU Li-hua;XIA Min;CHEN Yan-chao;WANG Qi-ming;YU Hua zhong;RAO Li-qun(College of Bioscience and Biotechnology.Hunan Agricultural University,Changsha 410128.China;Key Laboralory of Forestry Chemieal Engineering of Jishou University,Zhangjiajie,Hunan 427000,China)

机构地区：[1]湖南农业大学生物科学技术学院,长沙410128 [2]吉首大学林产化工湖南省重点实验室,湖南张家界427000

出　　处：《西南农业学报》2024年第11期2392-2401,共10页Southwest China Journal of Agricultural Sciences

基　　金：湖南省发改委科技专项(长财建指[2016]245号)。

摘　　要：【目的】分析比较显齿蛇葡萄与广东蛇葡萄的转录组序列,挖掘与二氢杨梅素(Dihydromyricetin,DHM)积累相关的基因,为探究显齿蛇葡萄高DHM积累机制提供新思路与方法。【方法】以显齿蛇葡萄和广东蛇葡萄叶片为材料,采用高效液相色谱法(HPLC)分析2种材料DHM含量,并利用RNA-Seq和qRT-PCR技术,分析DHM代谢途径相关基因的表达模式变化。【结果】显齿蛇葡萄与广东蛇葡萄的幼叶中DHM含量差异高达15倍,显齿蛇葡萄幼叶干重下DHM含量为336.8 mg/g,而广东蛇葡萄幼叶为20.8 mg/g。经转录组差异表达筛选,共获得14914个差异表达基因(Differentially expressed genes,DEGs),其中大量的DEGs显著富集到核糖体、糖酵解/糖异生、光合生物中的碳固定、苯丙烷生物合成、类黄酮生物合成等途径中。富集结果表明,显齿蛇葡萄在参与类黄酮合成代谢与分解代谢相关基因上与广东蛇葡萄有显著差异。GO富集分析结果表明,DEGs高度参与植物初生代谢过程,尤其是与核糖体相关的酶的合成与运输。分析发现类黄酮生物合成途径相关的AgCHS、AgCHI、AgF3’H、AgF3’5’H基因表达量高,且上调显著,推测可能是显齿蛇葡萄DHM积累的关键基因。此外,在DHM分解代谢途径中,CYP81E基因表达量显著下调,推测CYP81E的低表达可能是DHM高积累的原因之一。【结论】显齿蛇葡萄AgCHS、AgCHI、AgF3’H、AgF3’5’H等基因上调表达,AgCYP81E基因下调表达,能促进二氢杨梅素在显齿蛇葡萄叶片中的积累。本研究为药用植物次生代谢物积累的调控和培育高质量药材提供新的思路与途径。【Objective】By analyzing and comparing the transcriptome sequences of Ampelopsis grossedentata and A.cantoniensis,the genes related to the accumulation of dihydromyricetin(DHM)were excavated,which provided new ideas and methods for exploring the high DHM accumulation mechanism of A.grossedentata.【Method】The leaves of A.grossedentata and A.cantoniensis were used as materials,and the DHM content of the two materials was analyzed by high performance liquid chromatography(HPLC).RNA-Seq and qRT-PCR techniques were used to analyze the expression patterns of genes related to DHM metabolic pathway.【Result】The difference in DHM content in the young leaves of A.grossedentata and A.cantoniensis could be as high as 15 times,with DHM content of 336.8 mg/g in young leaves of A.grossedentata and 20.8 mg/g in A.cantoniensis.Through differential expression screening of the transcriptome,a total of 14914 differentially expressed genes(DEGs)were obtained,and a large number of DEGs were significantly enriched in pathways such as ribosome,glycolysis/gluconeogenesis,carbon fixation in photosynthetic organisms,phenylpropanoid biosynthesis and flavonoid biosynthesis.Enrichment results showed that there were significant differences in the genes involved in flavonoid synthesis and catabolism between A.grossedentata and A.cantoniensis.The results of GO enrichment analysis showed that DEGs were highly involved in the primary metabolic process of plants,especially the synthesis and transport of ribosome-related enzymes.In the study,it was found that the expression levels of AgCHS,AgCHI,AgF3'H and AgF3'5'H genes were high and significantly upregulated,suggesting that these genes were key genes in the flavonoid biosynthesis pathway of A.grossedentata DHM accumulation.In addition,in the DHM degradation pathway,the expression of the CYP81E gene was significantly downregulated,suggesting that the low expression of this gene might be one of the reasons for the high accumulation of DHM.In addition,in the DHM degradation pathway,the expre

关 键 词：显齿蛇葡萄 广东蛇葡萄 转录组测序 二氢杨梅素 积累机制 

分 类 号：S663.1[农业科学—果树学]