不同16S rRNA引物在溺死组织气单胞菌检测中的性能评估  

Comparison of different 16S rRNA primers for the detection of Aeromonas in drowned tissues

作　　者：宁浩冉 代昌海 种法国 王建鑫 王雅萱 胡洋洋 王业全[1,2] 张磊磊 张国安[1,2] NING Haoran;DAI Changhai;CHONG Faguo;WANG Jianxin;WANG Yaxuan;HU Yangyang;WANG Yequan;ZHANG Leilei;ZHANG Guoan(School of Forensic Medicine and Forensic Science,Jining Medical University,Jining 272067,China;Jining Key Laboratory of Forensic Medicine,Jining 272067,China;Physical Evidence Identification Center of Shandong Provincial Public Security Bureau,Jinan 250011,China)

机构地区：[1]济宁医学院法医学院,济宁272067 [2]济宁市法医学重点实验室,济宁272067 [3]山东省公安厅物证鉴定中心,济南250011

出　　处：《济宁医学院学报》2025年第1期8-11,共4页Journal of Jining Medical University

基　　金：山东省重点研发计划(重大科技创新工程)项目(2021CXGC011305);济宁医学院大学生创新创业训练计划项目(cx2023122z)。

摘　　要：目的 分析16S rRNA V3区引物、V4区引物和V3-V4区引物检测溺死组织中气单胞菌的差异。方法 采用PCR-扩增子测序方法,评估3种16S rRNA引物在5例溺死者的11个脏器中检测细菌DNA的能力,特别是气单胞菌DNA的检测效果。结果 相比于其他2个引物,V3引物在检测细菌分类操作单元(Operational Taxonomic Unit, OTU)方面更具优势,检测到的细菌OTU数量更多。测序结果分析表明,V3引物能够检测更丰富的细菌门(Phylum)和科(Family),以及更多的气单胞菌。结论 16S rRNA V3引物在检测溺死脏器中的气单胞菌可能更具优势,对溺死诊断具有潜在应用价值。Objective To analyze the performance of 16S rRNA V3-region primers,V4-region primers and V3-V4-region primers in detecting Aeromonas in drowned tissues.Methods The performance of three 16S rRNA primers was evaluated using PCR-amplifier sequencing to detect microorganisms,including Aeromonas,in the DNA of 11 organs from five drowned individuals.Results Compared with the other two primers,the V3 primer detected more bacterial classification Operational Taxonomic Unit(OTU).Sequencing results showed that the V3 primer detected a greater diversity of bacterial phyla and families,as well as most Aeromonas.Conclusion The V316S rRNA primers may be more effective for detecting Aeromonas in drowned organs,thus has potential application value in the diagnosis of drowning.

关 键 词：溺死 16S rRNA测序 气单胞菌 

分 类 号：R895[医药卫生—法医学]